Mechanical characterisation of a hydrogel-based microfluidic device for tissue culture

Microfluidic devices have been widely used for cell studies. In this project, a hydrogel-based microfluidic device was designed to study the transformation of stem cells into cardiac cells. A Polydimethylsiloxane (PDMS) membrane was sandwiched between two patterned PDMS layers to form the device. Th...

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Main Author: Nur Hidayah Jumal.
Other Authors: Hayden Taylor
Format: Final Year Project
Language:English
Published: 2013
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Online Access:http://hdl.handle.net/10356/54649
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-546492023-03-04T19:10:01Z Mechanical characterisation of a hydrogel-based microfluidic device for tissue culture Nur Hidayah Jumal. Hayden Taylor School of Mechanical and Aerospace Engineering DRNTU::Engineering::Nanotechnology Microfluidic devices have been widely used for cell studies. In this project, a hydrogel-based microfluidic device was designed to study the transformation of stem cells into cardiac cells. A Polydimethylsiloxane (PDMS) membrane was sandwiched between two patterned PDMS layers to form the device. The stem cells were to be embedded into collagen and this mixture was to be injected into a central channel in the device. The device was then subjected to vacuum to produce deformations that would induce strain on the stem cells. Concrete rheological data for collagen was sparse and a mechanical characterisation of collagen was done through experimentation. The microfluidic device doubled up as a device for the collagen characterisation and fluorescent microspheres were embedded in the collagen in place of stem cells. The fluorescent microspheres were tracked under a fluorescent microscope, and a graph of the microsphere locations was plotted against time. Data from the graph was used to determine the stiffness of the collagen. Simultaneously, a finite element analysis was done on the microfluidic device to study the feasibility of the design. The results showed that the device design was feasible for low values of collagen stiffness but the device would fail when the stiffness approached the stiffness of PDMS. Bachelor of Engineering (Mechanical Engineering) 2013-07-01T03:27:42Z 2013-07-01T03:27:42Z 2013 2013 Final Year Project (FYP) http://hdl.handle.net/10356/54649 en Nanyang Technological University 64 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Nanotechnology
spellingShingle DRNTU::Engineering::Nanotechnology
Nur Hidayah Jumal.
Mechanical characterisation of a hydrogel-based microfluidic device for tissue culture
description Microfluidic devices have been widely used for cell studies. In this project, a hydrogel-based microfluidic device was designed to study the transformation of stem cells into cardiac cells. A Polydimethylsiloxane (PDMS) membrane was sandwiched between two patterned PDMS layers to form the device. The stem cells were to be embedded into collagen and this mixture was to be injected into a central channel in the device. The device was then subjected to vacuum to produce deformations that would induce strain on the stem cells. Concrete rheological data for collagen was sparse and a mechanical characterisation of collagen was done through experimentation. The microfluidic device doubled up as a device for the collagen characterisation and fluorescent microspheres were embedded in the collagen in place of stem cells. The fluorescent microspheres were tracked under a fluorescent microscope, and a graph of the microsphere locations was plotted against time. Data from the graph was used to determine the stiffness of the collagen. Simultaneously, a finite element analysis was done on the microfluidic device to study the feasibility of the design. The results showed that the device design was feasible for low values of collagen stiffness but the device would fail when the stiffness approached the stiffness of PDMS.
author2 Hayden Taylor
author_facet Hayden Taylor
Nur Hidayah Jumal.
format Final Year Project
author Nur Hidayah Jumal.
author_sort Nur Hidayah Jumal.
title Mechanical characterisation of a hydrogel-based microfluidic device for tissue culture
title_short Mechanical characterisation of a hydrogel-based microfluidic device for tissue culture
title_full Mechanical characterisation of a hydrogel-based microfluidic device for tissue culture
title_fullStr Mechanical characterisation of a hydrogel-based microfluidic device for tissue culture
title_full_unstemmed Mechanical characterisation of a hydrogel-based microfluidic device for tissue culture
title_sort mechanical characterisation of a hydrogel-based microfluidic device for tissue culture
publishDate 2013
url http://hdl.handle.net/10356/54649
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