Multiplex gene array and immunohistochemical analysis with clinical pathological correlation for lung sarcomatoid carcinoma.

• Main Author: Zhuang, Yan.
• Other Authors: School of Biological Sciences
• Format: Final Year Project
• Language: English
• Published: 2013
• Subjects: DRNTU::Science::Biological sciences
• Online Access: http://hdl.handle.net/10356/54791
• Institution: Nanyang Technological University

Lung sarcomatoid carcinoma (SC) is a subtype of non-small cell lung carcinoma (NSCLC), comprising of five subtypes: pleomorphic carcinoma (PC), spindle cell carcinoma (SpCC), giant cell carcinoma, carcinosarcoma and pulmonary blastoma. Clinical features of 37 lung SC (PC and SpCC) samples were analyzed and they showed features similar to that of squamous cell carcinoma (SQCC). Immunohistochemistry (IHC) and Sequenom multiplex gene array were used in this study to analyze the IHC and molecular profiles of 19 SC resection samples. IHC result showed that the following markers were positive in our samples: TTF1, napsin, p63, CK5/6, MNF116, MSA, SMA, S100, vimentin, calponin, caldesmon and ALK. Coexpression of adenocarcinoma, SQCC and stromal markers was detected in our PC series. IHC profiling also showed the following markers to be overexpressed in PC: FGFR2 (75%), HGF (63%) and c-Met (13%). Molecular analysis of the 19 SC samples showed mutations in EGFR, EPHA5, NTRK2, PIK3CA, NOTCH1, TP53, EPHA3, MET, KRAS, STK11 and BRAF, which EGFR showed highest mutation rate (47%). Our results demonstrated that lung SC shares similar clinicopathological, IHC and molecular features with both adenocarcinoma and SQCC. This suggests that the carcinomatous origin of SC may be from a poorly differentiated NSCLC.