Bio and nano-engineered anode for high performance microbial fuel cell and its enhancement mechanism

This research focuses on developing novel nanostructured anode materials and engineered bacterial strains to significantly improve the bioelectrocatalytic efficiency and power density of microbial fuel cells (MFCs), and meanwhile exploring the fundamental insights of bacterial electron transfer duri...

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Main Author: Liu, Jing
Other Authors: Tan Thatt Yang Timothy
Format: Theses and Dissertations
Language:English
Published: 2014
Subjects:
Online Access:http://hdl.handle.net/10356/55769
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-557692023-03-03T16:03:10Z Bio and nano-engineered anode for high performance microbial fuel cell and its enhancement mechanism Liu, Jing Tan Thatt Yang Timothy School of Chemical and Biomedical Engineering DRNTU::Engineering::Bioengineering This research focuses on developing novel nanostructured anode materials and engineered bacterial strains to significantly improve the bioelectrocatalytic efficiency and power density of microbial fuel cells (MFCs), and meanwhile exploring the fundamental insights of bacterial electron transfer during the bioelectrocatalytic process. The first approach was to develop a new anode by electrochemically depositing graphene on carbon cloth for a Pseudomonas aeruginosa (P. aeruginosa) mediatorless MFC. The graphene modification improved power density and energy conversion efficiency by 2.7 and 3 times, respectively. To further increase the contact area between bacteria and graphene materials, a novel three-dimensional chitosan/vacuum stripped graphene (CHI/VSG) scaffold with hierarchically porous structure was carefully designed and successfully prepared as anode, which fulfills a remarkable 78 times maximum powder density improvement than carbon cloth anode. Furthermore, a facile bacteria-treatment approach of "perforating" pores and channels on the bacterial membrane was successfully developed to significantly improve the electron transfer rate between bacteria and electrode. In addition, we constructed an arcA knockout mutant Escherichia coli (arcA−) strain which shows enhanced activation of the citric acid cycle for efficient glycerol oxidation under microaerobic condition and excretes an endogenous mediator, resulting in much higher power density than its parental strain. The success of this project advances our knowledge about the electron transfer process and in-depth understanding of the bioelectricity production, and also provides new solutions for future MFCs. Doctor of Philosophy 2014-03-27T12:23:56Z 2014-03-27T12:23:56Z 2014 2014 Thesis http://hdl.handle.net/10356/55769 en 140 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Bioengineering
spellingShingle DRNTU::Engineering::Bioengineering
Liu, Jing
Bio and nano-engineered anode for high performance microbial fuel cell and its enhancement mechanism
description This research focuses on developing novel nanostructured anode materials and engineered bacterial strains to significantly improve the bioelectrocatalytic efficiency and power density of microbial fuel cells (MFCs), and meanwhile exploring the fundamental insights of bacterial electron transfer during the bioelectrocatalytic process. The first approach was to develop a new anode by electrochemically depositing graphene on carbon cloth for a Pseudomonas aeruginosa (P. aeruginosa) mediatorless MFC. The graphene modification improved power density and energy conversion efficiency by 2.7 and 3 times, respectively. To further increase the contact area between bacteria and graphene materials, a novel three-dimensional chitosan/vacuum stripped graphene (CHI/VSG) scaffold with hierarchically porous structure was carefully designed and successfully prepared as anode, which fulfills a remarkable 78 times maximum powder density improvement than carbon cloth anode. Furthermore, a facile bacteria-treatment approach of "perforating" pores and channels on the bacterial membrane was successfully developed to significantly improve the electron transfer rate between bacteria and electrode. In addition, we constructed an arcA knockout mutant Escherichia coli (arcA−) strain which shows enhanced activation of the citric acid cycle for efficient glycerol oxidation under microaerobic condition and excretes an endogenous mediator, resulting in much higher power density than its parental strain. The success of this project advances our knowledge about the electron transfer process and in-depth understanding of the bioelectricity production, and also provides new solutions for future MFCs.
author2 Tan Thatt Yang Timothy
author_facet Tan Thatt Yang Timothy
Liu, Jing
format Theses and Dissertations
author Liu, Jing
author_sort Liu, Jing
title Bio and nano-engineered anode for high performance microbial fuel cell and its enhancement mechanism
title_short Bio and nano-engineered anode for high performance microbial fuel cell and its enhancement mechanism
title_full Bio and nano-engineered anode for high performance microbial fuel cell and its enhancement mechanism
title_fullStr Bio and nano-engineered anode for high performance microbial fuel cell and its enhancement mechanism
title_full_unstemmed Bio and nano-engineered anode for high performance microbial fuel cell and its enhancement mechanism
title_sort bio and nano-engineered anode for high performance microbial fuel cell and its enhancement mechanism
publishDate 2014
url http://hdl.handle.net/10356/55769
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