Characterization of N-WASP in cell-adhesion and regulation of filopodia induction through WIREIRSP53 complex.
259 p.
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2014
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sg-ntu-dr.10356-574122023-02-28T18:41:31Z Characterization of N-WASP in cell-adhesion and regulation of filopodia induction through WIREIRSP53 complex. Ashish Misra Thirumaran s/o Thanabalu School of Biological Sciences DRNTU::Science::Biological sciences 259 p. The actin cytoskeleton, made of polymerized actin together with the actin associated proteins, plays a critical role in many cellular processes, including cell adhesion and cell motility. The Arp2/3 complex, made up of two actin related proteins, promotes actin polymerization by providing the nucleus. The Arp2/3 complex is active ated by the WASP family of proteins: WASP, N-WASP, WAVE1, WAVE2, and WAVE3. N-WASP (Neural Wiskott Aldrich Syndrome Protein) interacts with the mammalian verprolin family of proteins, W1P (WASP Interacting Protein), CR16 (Corticoid Regulated), WIRE (WIp-RElated) and regulates the actin cytoskeleton. N-WASP promotes the formation of actin-rich structures such as filopodia which plays critical roles in cell adhesion and cell motility. Analysis of the adhesion properties of NWASP+/+ and N-WASP-/- mouse embryonic fibroblasts to extracellular matrix proteins revealed that N-WASP is critical for cell adhesion to fibronectin. There was no significant difference in the localization of paxillin in the two cell lines, however the Vinculin patches in WASP+/+ cells were thicker and more prominent than those in N-WASP-/- cells. The ß1 integrins in N-WASP+/ cells were found in large clusters, while ß1 integrins were more dispersed in N-WASP-/- cells. The N-WASP-/- cells migrated more rapidly than N-WASP+/+ cells in a scratch migration assay. Thus, the data suggest that N-WASP deficiency leads to reduced adhesion to fibronectin and increased cell motility. Doctor of Philosophy (SBS) 2014-04-07T10:30:42Z 2014-04-07T10:30:42Z 2011 2011 Thesis http://hdl.handle.net/10356/57412 Nanyang Technological University application/pdf |
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DRNTU::Science::Biological sciences Ashish Misra Characterization of N-WASP in cell-adhesion and regulation of filopodia induction through WIREIRSP53 complex. |
| description |
259 p. |
| author2 |
Thirumaran s/o Thanabalu |
| author_facet |
Thirumaran s/o Thanabalu Ashish Misra |
| format |
Theses and Dissertations |
| author |
Ashish Misra |
| author_sort |
Ashish Misra |
| title |
Characterization of N-WASP in cell-adhesion and regulation of filopodia induction through WIREIRSP53 complex. |
| title_short |
Characterization of N-WASP in cell-adhesion and regulation of filopodia induction through WIREIRSP53 complex. |
| title_full |
Characterization of N-WASP in cell-adhesion and regulation of filopodia induction through WIREIRSP53 complex. |
| title_fullStr |
Characterization of N-WASP in cell-adhesion and regulation of filopodia induction through WIREIRSP53 complex. |
| title_full_unstemmed |
Characterization of N-WASP in cell-adhesion and regulation of filopodia induction through WIREIRSP53 complex. |
| title_sort |
characterization of n-wasp in cell-adhesion and regulation of filopodia induction through wireirsp53 complex. |
| publishDate |
2014 |
| url |
http://hdl.handle.net/10356/57412 |
| _version_ |
1759855790138589184 |