Synthesis of functionalized polymer nano-particles as fluorescent probes for bio-imaging
The objective was to characterise and optimise the desolvation process of Bovine Serum Albumin (BSA) for the preparation of nano-particles for bio-imaging applications. Following on the desolvation process of protein, the resulting nano-particles were stabilised by the addition of varying amounts of...
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| Format: | Final Year Project |
| Language: | English |
| Published: |
2014
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| Online Access: | http://hdl.handle.net/10356/60178 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | The objective was to characterise and optimise the desolvation process of Bovine Serum Albumin (BSA) for the preparation of nano-particles for bio-imaging applications. Following on the desolvation process of protein, the resulting nano-particles were stabilised by the addition of varying amounts of glutaraldehyde(cross-linker).
This report directed towards the synthesis of functionalized polymer nanoparticles as fluorescent probes for bio-imaging. Hence, number of method on the synthesis of fluorescence loaded BSA NPs were carried out.
Synthesis of fluorescence loaded BSA NPs were carried out using the 3 step process, sonication, cross-linking and THF removal. Other parameter including reducing particle size, stirring process, using ethanol as a desolvating agent, abosorption and emission analysis were also carried out.
The result has shown that using THF as a desolvating agent produce a particle size of less than 200nm. The result obtained implies that the brightness of the fluorescent-doped NPs is proportional to the number of encapsulated dye molecules. |
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