Characterization of gelatin-alginate hydrogel with 3D bioprinter

Burns is a serious public health problem which involves damage from the epidermis layer to the dermis layer of the skin. In most cases, damage beyond superficial dermal wounds is expected to undergo conventional excision and grafting surgery to expedite patient’s recovery process, prevent infection...

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Main Author: Ow, Chao Wei
Other Authors: Xu Chenjie
Format: Final Year Project
Language:English
Published: 2014
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Online Access:http://hdl.handle.net/10356/60245
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-602452023-03-03T15:39:29Z Characterization of gelatin-alginate hydrogel with 3D bioprinter Ow, Chao Wei Xu Chenjie School of Chemical and Biomedical Engineering DRNTU::Engineering Burns is a serious public health problem which involves damage from the epidermis layer to the dermis layer of the skin. In most cases, damage beyond superficial dermal wounds is expected to undergo conventional excision and grafting surgery to expedite patient’s recovery process, prevent infection and scarring. For cases where donor sites are limited, alternative treatments such as cultured epithelial autografts will be recommended to the patient. However, this technology still faces some drawbacks, such as poor take rate in full-thickness bed or in chronically infected area, delays in obtaining the grafts, sensitive to infection and high cost. With new emerging technologies such as bioprinting, replicating the ideal artificial skin that mimics the biological function of the human skin will not be too far off. In this study, pressure-assisted syringe based bioprinter is used in conjunction with gelatin-alginate hydrogel with the shape/structural integrity and viability of encapsulated HS-5 cells investigated. Results have shown that gelatin-alginate hydrogel with gelatin 6% and alginate 5% gives the best shape integrity using a set of constant bioprinter parameters. Furthermore, cell study was carried out and showed that the encapsulated cells proliferate with initial cell seeding density of 1.0 x 106 cells/mL while cell spreading was apparent when using initial cell seeding density of 4.0 x 106 cells/mL. Overall results show that the hybrid hydrogel when used together with pressure assisted syringe based bioprinter is a promising strategy towards tissue engineering, such as skin regeneration. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2014-05-26T03:46:23Z 2014-05-26T03:46:23Z 2014 2014 Final Year Project (FYP) http://hdl.handle.net/10356/60245 en Nanyang Technological University 53 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering
spellingShingle DRNTU::Engineering
Ow, Chao Wei
Characterization of gelatin-alginate hydrogel with 3D bioprinter
description Burns is a serious public health problem which involves damage from the epidermis layer to the dermis layer of the skin. In most cases, damage beyond superficial dermal wounds is expected to undergo conventional excision and grafting surgery to expedite patient’s recovery process, prevent infection and scarring. For cases where donor sites are limited, alternative treatments such as cultured epithelial autografts will be recommended to the patient. However, this technology still faces some drawbacks, such as poor take rate in full-thickness bed or in chronically infected area, delays in obtaining the grafts, sensitive to infection and high cost. With new emerging technologies such as bioprinting, replicating the ideal artificial skin that mimics the biological function of the human skin will not be too far off. In this study, pressure-assisted syringe based bioprinter is used in conjunction with gelatin-alginate hydrogel with the shape/structural integrity and viability of encapsulated HS-5 cells investigated. Results have shown that gelatin-alginate hydrogel with gelatin 6% and alginate 5% gives the best shape integrity using a set of constant bioprinter parameters. Furthermore, cell study was carried out and showed that the encapsulated cells proliferate with initial cell seeding density of 1.0 x 106 cells/mL while cell spreading was apparent when using initial cell seeding density of 4.0 x 106 cells/mL. Overall results show that the hybrid hydrogel when used together with pressure assisted syringe based bioprinter is a promising strategy towards tissue engineering, such as skin regeneration.
author2 Xu Chenjie
author_facet Xu Chenjie
Ow, Chao Wei
format Final Year Project
author Ow, Chao Wei
author_sort Ow, Chao Wei
title Characterization of gelatin-alginate hydrogel with 3D bioprinter
title_short Characterization of gelatin-alginate hydrogel with 3D bioprinter
title_full Characterization of gelatin-alginate hydrogel with 3D bioprinter
title_fullStr Characterization of gelatin-alginate hydrogel with 3D bioprinter
title_full_unstemmed Characterization of gelatin-alginate hydrogel with 3D bioprinter
title_sort characterization of gelatin-alginate hydrogel with 3d bioprinter
publishDate 2014
url http://hdl.handle.net/10356/60245
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