Direct real-time taqman PCR and PCR methods for bacterial pathogens in human blood samples

• Main Author: Koh, Yong Chuan
• Other Authors: Ayi Teck Choon @ Yeo Teck Choon
• Format: Final Year Project
• Language: English
• Published: 2014
• Subjects: DRNTU::Science::Biological sciences::Molecular biology
• Online Access: http://hdl.handle.net/10356/60729
• Institution: Nanyang Technological University

Traditional clinical diagnosis of bacterial pathogens involves cell culturing which could take days. Direct Polymerase Chain Reaction (Direct PCR) is a new method in detecting pathogens by directly putting samples into PCR without DNA extraction. However, not much work has been done for Direct real-...