Bioconjugated plasmonic gold nanorod vesicles for cancer cell imaging and therapy

The surfaces of gold nanorods synthesized by modified seed-mediated growth method were modified by polymer brushes, PLA and PEG to enhance the stability. Gold nanorods further self-assembled into plasmonic vesicles and were conjugated with monoclonal antibodies, namely anti-EpCAM to specifically tar...

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Main Author: Lim, Hui Ping
Other Authors: School of Chemical and Biomedical Engineering
Format: Final Year Project
Language:English
Published: 2014
Subjects:
Online Access:http://hdl.handle.net/10356/60941
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-609412023-03-03T15:39:40Z Bioconjugated plasmonic gold nanorod vesicles for cancer cell imaging and therapy Lim, Hui Ping School of Chemical and Biomedical Engineering Duan Hongwei DRNTU::Engineering::Bioengineering The surfaces of gold nanorods synthesized by modified seed-mediated growth method were modified by polymer brushes, PLA and PEG to enhance the stability. Gold nanorods further self-assembled into plasmonic vesicles and were conjugated with monoclonal antibodies, namely anti-EpCAM to specifically target liver cancer cell line known as Hep3B which overexpressed EpCAM cell surface receptors. Optical properties of bioconjugated plasmonic gold nanorod vesicles were investigated and demonstrated through live cell imaging and photothermal therapy which proven the multifunctional roles of nanoparticles in biomedical applications. As the longitudinal band of the self-assembled bioconjugated plasmonic gold nanorod vesicles fell in the NIR region where tissues absorption by blood and tissues were the least, they were able to scatter light strongly when uptake by Hep3B cells. Therefore by fine tuning of the aspect ratio, scattered light of different colours can be observed. This in turn contributed to bioimaging as it helps to distinguish cancerous cells. On the other hand, upon exposure to laser irradiation, bioconjugated plasmonic gold nanorod vesicles effectively converted light energy to heat energy and aid in localized heating effect which selectively killed Hep3B cells while leaving surrounding healthy cells unharmed. Additionally, future outlook of nanoparticles were also discussed so to bring nanotechnology to the next breakthrough. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2014-06-03T06:35:13Z 2014-06-03T06:35:13Z 2014 2014 Final Year Project (FYP) http://hdl.handle.net/10356/60941 en Nanyang Technological University 51 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Bioengineering
spellingShingle DRNTU::Engineering::Bioengineering
Lim, Hui Ping
Bioconjugated plasmonic gold nanorod vesicles for cancer cell imaging and therapy
description The surfaces of gold nanorods synthesized by modified seed-mediated growth method were modified by polymer brushes, PLA and PEG to enhance the stability. Gold nanorods further self-assembled into plasmonic vesicles and were conjugated with monoclonal antibodies, namely anti-EpCAM to specifically target liver cancer cell line known as Hep3B which overexpressed EpCAM cell surface receptors. Optical properties of bioconjugated plasmonic gold nanorod vesicles were investigated and demonstrated through live cell imaging and photothermal therapy which proven the multifunctional roles of nanoparticles in biomedical applications. As the longitudinal band of the self-assembled bioconjugated plasmonic gold nanorod vesicles fell in the NIR region where tissues absorption by blood and tissues were the least, they were able to scatter light strongly when uptake by Hep3B cells. Therefore by fine tuning of the aspect ratio, scattered light of different colours can be observed. This in turn contributed to bioimaging as it helps to distinguish cancerous cells. On the other hand, upon exposure to laser irradiation, bioconjugated plasmonic gold nanorod vesicles effectively converted light energy to heat energy and aid in localized heating effect which selectively killed Hep3B cells while leaving surrounding healthy cells unharmed. Additionally, future outlook of nanoparticles were also discussed so to bring nanotechnology to the next breakthrough.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Lim, Hui Ping
format Final Year Project
author Lim, Hui Ping
author_sort Lim, Hui Ping
title Bioconjugated plasmonic gold nanorod vesicles for cancer cell imaging and therapy
title_short Bioconjugated plasmonic gold nanorod vesicles for cancer cell imaging and therapy
title_full Bioconjugated plasmonic gold nanorod vesicles for cancer cell imaging and therapy
title_fullStr Bioconjugated plasmonic gold nanorod vesicles for cancer cell imaging and therapy
title_full_unstemmed Bioconjugated plasmonic gold nanorod vesicles for cancer cell imaging and therapy
title_sort bioconjugated plasmonic gold nanorod vesicles for cancer cell imaging and therapy
publishDate 2014
url http://hdl.handle.net/10356/60941
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