Microfluidic chip development for bio-defence application

The developments in Microfluidic applications have enabled a breakthrough in the creation of Lab-on-chip devices which allows rapid bio-chemical analysis for fast diagnostics. These chips give significant benefits in terms of low sample volume, less sample wastage, fast analysis time, cost effective...

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Main Author: Sim, Bernard Wee Jun
Other Authors: Li King Ho Holden
Format: Final Year Project
Language:English
Published: 2014
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Online Access:http://hdl.handle.net/10356/60986
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-609862023-03-04T18:32:37Z Microfluidic chip development for bio-defence application Sim, Bernard Wee Jun Li King Ho Holden School of Mechanical and Aerospace Engineering DRNTU::Engineering The developments in Microfluidic applications have enabled a breakthrough in the creation of Lab-on-chip devices which allows rapid bio-chemical analysis for fast diagnostics. These chips give significant benefits in terms of low sample volume, less sample wastage, fast analysis time, cost effectiveness as well as the possibility of developing different diagnostic on different chips all at one go. Hence, in this report, it focuses on the development of a novel idea of using a magnetic membrane to create a micromixer which could be used to introduce a turbulent flow to a fluid enhancing the mixing process. A structured research is then conducted, firstly, with the study, fabrication and characterisation of an electromagnet to enable a steady and constant magnetic flux to be produced. Secondly, the characterisation of Spin Speed vs. Membrane thickness using Polydimethylsiloxane (PDMS) is done, to enable the accurate prediction of membrane thickness during the actual creation of the magnetic membrane. Next, the actual membranes are created with different concentrations of Iron(III)Oxide cores as well as a 200 and 500 micron thick magnetic paper core. These cores are embedded between two 25 micron thick PDMS membranes to allow the membrane to keep its elasticity but at the same time deflect with an application of magnetic flux. Static and dynamic deflection tests are then conducted to characterise deflections using a microscope and high speed camera respectively. During the static deflection test, Iron(III)Oxide cores show no deflection and the 500 micron magnetic paper produced better deflection results as compared to the 200 micron magnetic paper. Hence, the 500 micron thick magnetic paper core was chosen to undergo the dynamic deflection testing which showed significant deflection of more than 200 microns dynamically at 100Hz. Fluorescent dye is then added to water within the well with and without actuation of the membrane to show the diffusion process and the enhanced mixing process respectively. Images are than being captured to prove the success in using the concept of the magnetic membrane for a micromixing application and finally, possible improvements and future works are listed to show the probable further research that could be carried out. Bachelor of Engineering (Mechanical Engineering) 2014-06-04T01:26:34Z 2014-06-04T01:26:34Z 2014 2014 Final Year Project (FYP) http://hdl.handle.net/10356/60986 en Nanyang Technological University 97 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering
spellingShingle DRNTU::Engineering
Sim, Bernard Wee Jun
Microfluidic chip development for bio-defence application
description The developments in Microfluidic applications have enabled a breakthrough in the creation of Lab-on-chip devices which allows rapid bio-chemical analysis for fast diagnostics. These chips give significant benefits in terms of low sample volume, less sample wastage, fast analysis time, cost effectiveness as well as the possibility of developing different diagnostic on different chips all at one go. Hence, in this report, it focuses on the development of a novel idea of using a magnetic membrane to create a micromixer which could be used to introduce a turbulent flow to a fluid enhancing the mixing process. A structured research is then conducted, firstly, with the study, fabrication and characterisation of an electromagnet to enable a steady and constant magnetic flux to be produced. Secondly, the characterisation of Spin Speed vs. Membrane thickness using Polydimethylsiloxane (PDMS) is done, to enable the accurate prediction of membrane thickness during the actual creation of the magnetic membrane. Next, the actual membranes are created with different concentrations of Iron(III)Oxide cores as well as a 200 and 500 micron thick magnetic paper core. These cores are embedded between two 25 micron thick PDMS membranes to allow the membrane to keep its elasticity but at the same time deflect with an application of magnetic flux. Static and dynamic deflection tests are then conducted to characterise deflections using a microscope and high speed camera respectively. During the static deflection test, Iron(III)Oxide cores show no deflection and the 500 micron magnetic paper produced better deflection results as compared to the 200 micron magnetic paper. Hence, the 500 micron thick magnetic paper core was chosen to undergo the dynamic deflection testing which showed significant deflection of more than 200 microns dynamically at 100Hz. Fluorescent dye is then added to water within the well with and without actuation of the membrane to show the diffusion process and the enhanced mixing process respectively. Images are than being captured to prove the success in using the concept of the magnetic membrane for a micromixing application and finally, possible improvements and future works are listed to show the probable further research that could be carried out.
author2 Li King Ho Holden
author_facet Li King Ho Holden
Sim, Bernard Wee Jun
format Final Year Project
author Sim, Bernard Wee Jun
author_sort Sim, Bernard Wee Jun
title Microfluidic chip development for bio-defence application
title_short Microfluidic chip development for bio-defence application
title_full Microfluidic chip development for bio-defence application
title_fullStr Microfluidic chip development for bio-defence application
title_full_unstemmed Microfluidic chip development for bio-defence application
title_sort microfluidic chip development for bio-defence application
publishDate 2014
url http://hdl.handle.net/10356/60986
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