Cell encapsulation in gelatin microspheres for tissue engineering

• Main Author: Li, JiaXin
• Other Authors: Wang Dongan
• Format: Theses and Dissertations
• Language: English
• Published: 2014
• Subjects: DRNTU::Engineering
• Online Access: http://hdl.handle.net/10356/61131
• Institution: Nanyang Technological University

Since the cell encapsulation technology has been used for many areas, such as cell delivery, cell culture and tissue engineering. This promising technique still undergoes development and improvement. Recently there is a new method for cell encapsulation by forming of temperature-cured dissolvable cell-laden gelatin microspheres. We expected that method could be used for fabricating BTC-6 constructs using this gelatin microspheres as intermediate. Before encapsulated BTC-6 cells, we did HepG2 cells encapsulation and got good results of cell viability and could form injectable cell islet, this result offered a premise for encapsulating BTC-6 cells. However after the experiment done on beta cells, the result was not so good, but this method still somehow can provide a suitable condition for beta cells proliferation. So future research should be focus optimizing the parameters in fabrication, such as the size of microspheres and concentration of cells. Cell viability was evaluated though WST-1, Live and dead.