Synthesis of magnetoliposomes and evaluating of their cell internalization by external magnetic field

While thermosensitive liposomes and magnetic nanoparticles are widely researched on by many pharmaceutical companies, magnetoliposomes show higher efficiency of triggering liposomal release at inner organs, than conventional ones. Thus, this project aims to develop a magnetic-sensitive drug delivery...

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Main Author: Lin, Jinjie
Other Authors: School of Chemical and Biomedical Engineering
Format: Final Year Project
Language:English
Published: 2014
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Online Access:http://hdl.handle.net/10356/61554
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-615542023-03-03T15:34:26Z Synthesis of magnetoliposomes and evaluating of their cell internalization by external magnetic field Lin, Jinjie School of Chemical and Biomedical Engineering Xu, Chenjie DRNTU::Engineering While thermosensitive liposomes and magnetic nanoparticles are widely researched on by many pharmaceutical companies, magnetoliposomes show higher efficiency of triggering liposomal release at inner organs, than conventional ones. Thus, this project aims to develop a magnetic-sensitive drug delivery system, coupled to widely used nanoparticles and liposomes, which could be adopted by these companies. Lysolipid (MPPC)-containing thermosensitive long circulating liposomes were synthesized from different formulations. The size and volume, and site of encapsulation, of magnetite (Fe3O4) nanoparticles entrapped, were varied. The thermal stability, and cytotoxicity effect on B16-F10 melanoma cell line, of the magnetoliposomes were studied. The size (hydrodynamic diameter) of the magnetoliposomes generally ranged from 100nm to 200nm, and showed high stability. Encapsulation of larger and lower volume of nanoparticles, within the liposomes’ cores, led to smaller magnetoliposomes. At 42oC and 48oC, the liposomal release efficiencies were high (76% and 100%, respectively). 1μl of magnetoliposomes/ml media resulted in low cytotoxicity (64.9% of cells remained viable). Bachelor of Engineering (Chemical and Biomolecular Engineering) 2014-06-11T07:36:44Z 2014-06-11T07:36:44Z 2014 2014 Final Year Project (FYP) http://hdl.handle.net/10356/61554 en Nanyang Technological University 72 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering
spellingShingle DRNTU::Engineering
Lin, Jinjie
Synthesis of magnetoliposomes and evaluating of their cell internalization by external magnetic field
description While thermosensitive liposomes and magnetic nanoparticles are widely researched on by many pharmaceutical companies, magnetoliposomes show higher efficiency of triggering liposomal release at inner organs, than conventional ones. Thus, this project aims to develop a magnetic-sensitive drug delivery system, coupled to widely used nanoparticles and liposomes, which could be adopted by these companies. Lysolipid (MPPC)-containing thermosensitive long circulating liposomes were synthesized from different formulations. The size and volume, and site of encapsulation, of magnetite (Fe3O4) nanoparticles entrapped, were varied. The thermal stability, and cytotoxicity effect on B16-F10 melanoma cell line, of the magnetoliposomes were studied. The size (hydrodynamic diameter) of the magnetoliposomes generally ranged from 100nm to 200nm, and showed high stability. Encapsulation of larger and lower volume of nanoparticles, within the liposomes’ cores, led to smaller magnetoliposomes. At 42oC and 48oC, the liposomal release efficiencies were high (76% and 100%, respectively). 1μl of magnetoliposomes/ml media resulted in low cytotoxicity (64.9% of cells remained viable).
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Lin, Jinjie
format Final Year Project
author Lin, Jinjie
author_sort Lin, Jinjie
title Synthesis of magnetoliposomes and evaluating of their cell internalization by external magnetic field
title_short Synthesis of magnetoliposomes and evaluating of their cell internalization by external magnetic field
title_full Synthesis of magnetoliposomes and evaluating of their cell internalization by external magnetic field
title_fullStr Synthesis of magnetoliposomes and evaluating of their cell internalization by external magnetic field
title_full_unstemmed Synthesis of magnetoliposomes and evaluating of their cell internalization by external magnetic field
title_sort synthesis of magnetoliposomes and evaluating of their cell internalization by external magnetic field
publishDate 2014
url http://hdl.handle.net/10356/61554
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