Hydrogen/deuterium exchange mass spectrometry reveals distinct mechanisms of viral RNA recognition and activation by RIG-I like receptors
RIG-I like receptors (RLRs) are the major intracellular immune receptors that recognize viral RNA species, undergo a series of conformational transitions and activate the interferon-mediated antiviral response. However, full-length RLRs resist crystallographic efforts and a molecular description of...
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sg-ntu-dr.10356-629432023-02-28T18:41:09Z Hydrogen/deuterium exchange mass spectrometry reveals distinct mechanisms of viral RNA recognition and activation by RIG-I like receptors Zheng, Jie Tang Kai Liu Chuan Fa School of Biological Sciences BioSciences Research Centre DRNTU::Science::Biological sciences::Biophysics RIG-I like receptors (RLRs) are the major intracellular immune receptors that recognize viral RNA species, undergo a series of conformational transitions and activate the interferon-mediated antiviral response. However, full-length RLRs resist crystallographic efforts and a molecular description of their activation pathways remains hypothetical. Here we employ Hydrogen/deuterium exchange coupled with mass spectrometry (HDX-MS) to probe the apo states of RLRs and to dissect the molecular details with respect to RNA recognition, ATP hydrolysis and CARDs activation. Our data provide a high-resolution orthogonal view of RLR activation in solutions and offer new evidence for the molecular mechanism of RLR activation. DOCTOR OF PHILOSOPHY (SBS) 2015-05-04T04:21:28Z 2015-05-04T04:21:28Z 2015 2015 Thesis Zheng, J. (2015). Hydrogen/deuterium exchange mass spectrometry reveals distinct mechanisms of viral RNA recognition and activation by RIG-I like receptors. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/62943 10.32657/10356/62943 en 178 p. application/pdf |
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DRNTU::Science::Biological sciences::Biophysics Zheng, Jie Hydrogen/deuterium exchange mass spectrometry reveals distinct mechanisms of viral RNA recognition and activation by RIG-I like receptors |
| description |
RIG-I like receptors (RLRs) are the major intracellular immune receptors that recognize viral RNA species, undergo a series of conformational transitions and activate the interferon-mediated antiviral response. However, full-length RLRs resist crystallographic efforts and a molecular description of their activation pathways remains hypothetical. Here we employ Hydrogen/deuterium exchange coupled with mass spectrometry (HDX-MS) to probe the apo states of RLRs and to dissect the molecular details with respect to RNA recognition, ATP hydrolysis and CARDs activation. Our data provide a high-resolution orthogonal view of RLR activation in solutions and offer new evidence for the molecular mechanism of RLR activation. |
| author2 |
Tang Kai |
| author_facet |
Tang Kai Zheng, Jie |
| format |
Theses and Dissertations |
| author |
Zheng, Jie |
| author_sort |
Zheng, Jie |
| title |
Hydrogen/deuterium exchange mass spectrometry reveals distinct mechanisms of viral RNA recognition and activation by RIG-I like receptors |
| title_short |
Hydrogen/deuterium exchange mass spectrometry reveals distinct mechanisms of viral RNA recognition and activation by RIG-I like receptors |
| title_full |
Hydrogen/deuterium exchange mass spectrometry reveals distinct mechanisms of viral RNA recognition and activation by RIG-I like receptors |
| title_fullStr |
Hydrogen/deuterium exchange mass spectrometry reveals distinct mechanisms of viral RNA recognition and activation by RIG-I like receptors |
| title_full_unstemmed |
Hydrogen/deuterium exchange mass spectrometry reveals distinct mechanisms of viral RNA recognition and activation by RIG-I like receptors |
| title_sort |
hydrogen/deuterium exchange mass spectrometry reveals distinct mechanisms of viral rna recognition and activation by rig-i like receptors |
| publishDate |
2015 |
| url |
https://hdl.handle.net/10356/62943 |
| _version_ |
1759855685627019264 |