Surface manipulation using gold micro pattern to modulate stem cell behaviour

Various biophysical methods like substrate topography were used in the past to promote the specific cell activities like proliferation, differentiation. However, the effect of topography on myogenic lineage commitment of stem cell has not been widely exposed. In this project, we studied specific dif...

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Main Author: Arliando Setiadi
Other Authors: Tan Lay Poh
Format: Final Year Project
Language:English
Published: 2015
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Online Access:http://hdl.handle.net/10356/62999
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-629992023-03-04T15:39:35Z Surface manipulation using gold micro pattern to modulate stem cell behaviour Arliando Setiadi Tan Lay Poh School of Materials Science and Engineering DRNTU::Engineering::Materials Various biophysical methods like substrate topography were used in the past to promote the specific cell activities like proliferation, differentiation. However, the effect of topography on myogenic lineage commitment of stem cell has not been widely exposed. In this project, we studied specific differentiation fate of human mesenchymal stem cells (hMSCs) grown on biofunctionalized gold micropattern in order to understand the cell-material interaction and its effect on the cell differentiation fate. The hMSCs were seeded on the bio-functionalized gold micropattern substrate and plain gold coated substrate which acted as the control. The patterned cells showed well-organized and aligned actin filaments network. In contrast, randomly distributed actin filaments network was observed in cells grown plain gold coated substrate. To identify the stem cell differentiation fate, a mature marker of myogenic lineage [β-myosin heavy chain (MHC)] was used. The immunostaining results revealed that β-MHC was predominantly expressed in patterned cells only while no MHC expression was observed in unpatterned cells. This study demonstrated that our novel biofunctionalized gold micropattern platform has ability to induce myogenesis in stem cells and can be used to form the terminally differentiated myotubes in future. Bachelor of Engineering (Materials Engineering) 2015-05-05T02:02:21Z 2015-05-05T02:02:21Z 2015 2015 Final Year Project (FYP) http://hdl.handle.net/10356/62999 en Nanyang Technological University 34 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Materials
spellingShingle DRNTU::Engineering::Materials
Arliando Setiadi
Surface manipulation using gold micro pattern to modulate stem cell behaviour
description Various biophysical methods like substrate topography were used in the past to promote the specific cell activities like proliferation, differentiation. However, the effect of topography on myogenic lineage commitment of stem cell has not been widely exposed. In this project, we studied specific differentiation fate of human mesenchymal stem cells (hMSCs) grown on biofunctionalized gold micropattern in order to understand the cell-material interaction and its effect on the cell differentiation fate. The hMSCs were seeded on the bio-functionalized gold micropattern substrate and plain gold coated substrate which acted as the control. The patterned cells showed well-organized and aligned actin filaments network. In contrast, randomly distributed actin filaments network was observed in cells grown plain gold coated substrate. To identify the stem cell differentiation fate, a mature marker of myogenic lineage [β-myosin heavy chain (MHC)] was used. The immunostaining results revealed that β-MHC was predominantly expressed in patterned cells only while no MHC expression was observed in unpatterned cells. This study demonstrated that our novel biofunctionalized gold micropattern platform has ability to induce myogenesis in stem cells and can be used to form the terminally differentiated myotubes in future.
author2 Tan Lay Poh
author_facet Tan Lay Poh
Arliando Setiadi
format Final Year Project
author Arliando Setiadi
author_sort Arliando Setiadi
title Surface manipulation using gold micro pattern to modulate stem cell behaviour
title_short Surface manipulation using gold micro pattern to modulate stem cell behaviour
title_full Surface manipulation using gold micro pattern to modulate stem cell behaviour
title_fullStr Surface manipulation using gold micro pattern to modulate stem cell behaviour
title_full_unstemmed Surface manipulation using gold micro pattern to modulate stem cell behaviour
title_sort surface manipulation using gold micro pattern to modulate stem cell behaviour
publishDate 2015
url http://hdl.handle.net/10356/62999
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