Surface manipulation using gold micro pattern to modulate stem cell behaviour
Various biophysical methods like substrate topography were used in the past to promote the specific cell activities like proliferation, differentiation. However, the effect of topography on myogenic lineage commitment of stem cell has not been widely exposed. In this project, we studied specific dif...
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sg-ntu-dr.10356-629992023-03-04T15:39:35Z Surface manipulation using gold micro pattern to modulate stem cell behaviour Arliando Setiadi Tan Lay Poh School of Materials Science and Engineering DRNTU::Engineering::Materials Various biophysical methods like substrate topography were used in the past to promote the specific cell activities like proliferation, differentiation. However, the effect of topography on myogenic lineage commitment of stem cell has not been widely exposed. In this project, we studied specific differentiation fate of human mesenchymal stem cells (hMSCs) grown on biofunctionalized gold micropattern in order to understand the cell-material interaction and its effect on the cell differentiation fate. The hMSCs were seeded on the bio-functionalized gold micropattern substrate and plain gold coated substrate which acted as the control. The patterned cells showed well-organized and aligned actin filaments network. In contrast, randomly distributed actin filaments network was observed in cells grown plain gold coated substrate. To identify the stem cell differentiation fate, a mature marker of myogenic lineage [β-myosin heavy chain (MHC)] was used. The immunostaining results revealed that β-MHC was predominantly expressed in patterned cells only while no MHC expression was observed in unpatterned cells. This study demonstrated that our novel biofunctionalized gold micropattern platform has ability to induce myogenesis in stem cells and can be used to form the terminally differentiated myotubes in future. Bachelor of Engineering (Materials Engineering) 2015-05-05T02:02:21Z 2015-05-05T02:02:21Z 2015 2015 Final Year Project (FYP) http://hdl.handle.net/10356/62999 en Nanyang Technological University 34 p. application/pdf |
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DRNTU::Engineering::Materials Arliando Setiadi Surface manipulation using gold micro pattern to modulate stem cell behaviour |
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Various biophysical methods like substrate topography were used in the past to promote the specific cell activities like proliferation, differentiation. However, the effect of topography on myogenic lineage commitment of stem cell has not been widely exposed. In this project, we studied specific differentiation fate of human mesenchymal stem cells (hMSCs) grown on biofunctionalized gold micropattern in order to understand the cell-material interaction and its effect on the cell differentiation fate. The hMSCs were seeded on the bio-functionalized gold micropattern substrate and plain gold coated substrate which acted as the control. The patterned cells showed well-organized and aligned actin filaments network. In contrast, randomly distributed actin filaments network was observed in cells grown plain gold coated substrate. To identify the stem cell differentiation fate, a mature marker of myogenic lineage [β-myosin heavy chain (MHC)] was used. The immunostaining results revealed that β-MHC was predominantly expressed in patterned cells only while no MHC expression was observed in unpatterned cells. This study demonstrated that our novel biofunctionalized gold micropattern platform has ability to induce myogenesis in stem cells and can be used to form the terminally differentiated myotubes in future. |
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Tan Lay Poh |
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Tan Lay Poh Arliando Setiadi |
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Final Year Project |
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Arliando Setiadi |
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Arliando Setiadi |
title |
Surface manipulation using gold micro pattern to modulate stem cell behaviour |
title_short |
Surface manipulation using gold micro pattern to modulate stem cell behaviour |
title_full |
Surface manipulation using gold micro pattern to modulate stem cell behaviour |
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Surface manipulation using gold micro pattern to modulate stem cell behaviour |
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Surface manipulation using gold micro pattern to modulate stem cell behaviour |
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surface manipulation using gold micro pattern to modulate stem cell behaviour |
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2015 |
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http://hdl.handle.net/10356/62999 |
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1759855152556146688 |