Detection of pathogens in swimming pools

Waterborne outbreaks in swimming pools are persistent problems in the developed world. Although pools in Singapore have been reported to be free of Escherichia coli, no article was published to study and determine the microbes and potential pathogens that could be present in the water. In this study...

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Main Author: Loh, Ying Ru
Other Authors: Yang Liang
Format: Final Year Project
Language:English
Published: 2015
Subjects:
Online Access:http://hdl.handle.net/10356/63627
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-636272023-02-28T18:03:38Z Detection of pathogens in swimming pools Loh, Ying Ru Yang Liang School of Biological Sciences Singapore Centre for Environmental Life Sciences Engineering DRNTU::Science::Biological sciences::Microbiology::Drug Resistance Waterborne outbreaks in swimming pools are persistent problems in the developed world. Although pools in Singapore have been reported to be free of Escherichia coli, no article was published to study and determine the microbes and potential pathogens that could be present in the water. In this study, backwash effluent of a swimming pool was collected to study the presence of antibiotic resistant microbial community. Potential bacterial pathogens were identified using two approaches that would complement each other: culture independent and culture dependent. Bacterial genomes and large plasmids were extracted for sequencing in culture independent approach whereas for culture dependent approach, plating was conducted on antibiotic medium to culture the antibiotic resistant bacteria. Based on the culture dependent approach, Bacillus and Aeromonas were discovered to be the most abundant in the pool backwash effluent. Furthermore, there were various nosocomial bacteria like Chryseobacterium spp, Flavobacterium spp, and Stenotrophomonas maltophilia and novel bacteria like Algoriphagus shivajiensis and Elizabethkingia anopheles being isolated. The common causative agents which contaminate aquatic environments are gram-negative bacteria. Hence, these findings imply the presence of antibiotic – resistance microbes which are potential pathogen to human in the pool and a need for constant surveillance to prevent waterborne outbreaks. Bachelor of Science in Biological Sciences 2015-05-18T02:29:08Z 2015-05-18T02:29:08Z 2015 2015 Final Year Project (FYP) http://hdl.handle.net/10356/63627 en Nanyang Technological University 47 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology::Drug Resistance
spellingShingle DRNTU::Science::Biological sciences::Microbiology::Drug Resistance
Loh, Ying Ru
Detection of pathogens in swimming pools
description Waterborne outbreaks in swimming pools are persistent problems in the developed world. Although pools in Singapore have been reported to be free of Escherichia coli, no article was published to study and determine the microbes and potential pathogens that could be present in the water. In this study, backwash effluent of a swimming pool was collected to study the presence of antibiotic resistant microbial community. Potential bacterial pathogens were identified using two approaches that would complement each other: culture independent and culture dependent. Bacterial genomes and large plasmids were extracted for sequencing in culture independent approach whereas for culture dependent approach, plating was conducted on antibiotic medium to culture the antibiotic resistant bacteria. Based on the culture dependent approach, Bacillus and Aeromonas were discovered to be the most abundant in the pool backwash effluent. Furthermore, there were various nosocomial bacteria like Chryseobacterium spp, Flavobacterium spp, and Stenotrophomonas maltophilia and novel bacteria like Algoriphagus shivajiensis and Elizabethkingia anopheles being isolated. The common causative agents which contaminate aquatic environments are gram-negative bacteria. Hence, these findings imply the presence of antibiotic – resistance microbes which are potential pathogen to human in the pool and a need for constant surveillance to prevent waterborne outbreaks.
author2 Yang Liang
author_facet Yang Liang
Loh, Ying Ru
format Final Year Project
author Loh, Ying Ru
author_sort Loh, Ying Ru
title Detection of pathogens in swimming pools
title_short Detection of pathogens in swimming pools
title_full Detection of pathogens in swimming pools
title_fullStr Detection of pathogens in swimming pools
title_full_unstemmed Detection of pathogens in swimming pools
title_sort detection of pathogens in swimming pools
publishDate 2015
url http://hdl.handle.net/10356/63627
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