The role of bacteriophage in biofilm development of pseudomonas aeruginosa

Pseudomonas aeruginosa is an opportunistic pathogen which can develop biofilms. Biofilm of P. aeruginosa has shown to give rise to phenotypic variants. The factors that contribute to the formation of variants in P. aeruginosa has not been extensively studied. It has been shown that emergence of vari...

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Main Author: Shifana Raja Abdeen
Other Authors: Scott Rice
Format: Final Year Project
Language:English
Published: 2015
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Online Access:http://hdl.handle.net/10356/63946
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-639462023-02-28T18:03:38Z The role of bacteriophage in biofilm development of pseudomonas aeruginosa Shifana Raja Abdeen Scott Rice School of Biological Sciences DRNTU::Science::Biological sciences Pseudomonas aeruginosa is an opportunistic pathogen which can develop biofilms. Biofilm of P. aeruginosa has shown to give rise to phenotypic variants. The factors that contribute to the formation of variants in P. aeruginosa has not been extensively studied. It has been shown that emergence of variants corresponds with the detection of superinfective phage. Therefore, PAO1 (host) and superinfective-Pf4 (phage) were used as a model to study the effects of host-phage interaction on variant formations and virulence during biofilm development. In this study the transcriptome of PAO1 WT and PAO1ΔPf4 was monitored to test the effects of exposure to Pf4 in order to better understand the factors involved in variant formation. Upon infection of phage, genes involved in SOS response, were upregulated. As the polymerases involved in this response system tend to be error-prone, increase in mutation rates could result in formation of variants. Transcriptomic validation also showed that expression levels of pyoverdine genes in biofilms of PAO1 that harbours the Pf4 prophage. These findings indicate that activation of SOS response due to phage infection is a possible mechanism of variant formation in P. aeruginosa biofilm and pyoverdine might be one of the virulence factors regulated by the Pf4 phage. Bachelor of Science in Biological Sciences 2015-05-20T08:02:27Z 2015-05-20T08:02:27Z 2015 2015 Final Year Project (FYP) http://hdl.handle.net/10356/63946 en 40 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Shifana Raja Abdeen
The role of bacteriophage in biofilm development of pseudomonas aeruginosa
description Pseudomonas aeruginosa is an opportunistic pathogen which can develop biofilms. Biofilm of P. aeruginosa has shown to give rise to phenotypic variants. The factors that contribute to the formation of variants in P. aeruginosa has not been extensively studied. It has been shown that emergence of variants corresponds with the detection of superinfective phage. Therefore, PAO1 (host) and superinfective-Pf4 (phage) were used as a model to study the effects of host-phage interaction on variant formations and virulence during biofilm development. In this study the transcriptome of PAO1 WT and PAO1ΔPf4 was monitored to test the effects of exposure to Pf4 in order to better understand the factors involved in variant formation. Upon infection of phage, genes involved in SOS response, were upregulated. As the polymerases involved in this response system tend to be error-prone, increase in mutation rates could result in formation of variants. Transcriptomic validation also showed that expression levels of pyoverdine genes in biofilms of PAO1 that harbours the Pf4 prophage. These findings indicate that activation of SOS response due to phage infection is a possible mechanism of variant formation in P. aeruginosa biofilm and pyoverdine might be one of the virulence factors regulated by the Pf4 phage.
author2 Scott Rice
author_facet Scott Rice
Shifana Raja Abdeen
format Final Year Project
author Shifana Raja Abdeen
author_sort Shifana Raja Abdeen
title The role of bacteriophage in biofilm development of pseudomonas aeruginosa
title_short The role of bacteriophage in biofilm development of pseudomonas aeruginosa
title_full The role of bacteriophage in biofilm development of pseudomonas aeruginosa
title_fullStr The role of bacteriophage in biofilm development of pseudomonas aeruginosa
title_full_unstemmed The role of bacteriophage in biofilm development of pseudomonas aeruginosa
title_sort role of bacteriophage in biofilm development of pseudomonas aeruginosa
publishDate 2015
url http://hdl.handle.net/10356/63946
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