Design and fabrication of a microfluidics device for blood plasma separation

• Main Author: Ong, Eng Hui
• Other Authors: Gary Ng Sum Huan
• Format: Final Year Project
• Language: English
• Published: 2015
• Subjects: DRNTU::Engineering::Bioengineering
• Online Access: http://hdl.handle.net/10356/64098
• Institution: Nanyang Technological University

Microfluidics is a design and fabrication of system which involve in manipulating small amount of liquid. Due to small amount of sample is needed in microfluidic, there are advantages of using microfluidic in analysis such as faster response time and so on. Much research has been done on blood plasma separation using microfluidic means. There are several types of method in separating blood plasma from blood cells such as magnetism, centrifugal, electric field, etc. In this project, blood plasma separation using microfluidic incorporate with magnetism was done. In this project, a microfluidic device was design and fabricated. The microfluidic device was a PMMA chip with serpentine channel. A metal mesh which was used to create high gradient magnetic field (HGMF) was laser cut. A permanent magnet was used as the source of external magnetic field. The metal mesh was placed on top of the magnet followed up with the microfluidic device. The blood sample was loaded from the inlet connector and was pumped with syringe pump from the outlet. The blood plasma yield and blood plasma purity obtained from 1:10 diluted blood were 21.933 % and 100 % respectively. The blood plasma purity obtained from non diluted blood was 6.875 %. The time taken for the 1:10 diluted blood and non diluted blood to reach the outlet were about 5 minutes and about 9 minutes respectively.