Investigation on the microbial profile and waste stabilization during in situ aeration of landfill bioreactors at different temperature

In situ aeration has been a popular and promising method for the advantages it bring about such as reduction of emission of greenhouse gases, accelerating of waste stabilization and settlement, decrease in concentrations of leachate contaminants and being able to provide the optimal conditions for m...

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Main Author: Ng, Wei Hong
Other Authors: Wang Jing-Yuan
Format: Final Year Project
Language:English
Published: 2015
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Online Access:http://hdl.handle.net/10356/64236
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-642362023-03-03T17:13:10Z Investigation on the microbial profile and waste stabilization during in situ aeration of landfill bioreactors at different temperature Ng, Wei Hong Wang Jing-Yuan School of Civil and Environmental Engineering Residues and Resource Reclamation Centre DRNTU::Engineering::Environmental engineering::Waste management In situ aeration has been a popular and promising method for the advantages it bring about such as reduction of emission of greenhouse gases, accelerating of waste stabilization and settlement, decrease in concentrations of leachate contaminants and being able to provide the optimal conditions for microbial decomposition. The purpose of this study is to investigate and develop a better understanding of the influences of different temperature on the aeration performances and microbial communities. Hence, this give rises to our study using four Landfill Bioreactors (LBRs) NR35 (control), AR35, AR45 and AR55 under anaerobic (35°C), aerobic (35°C), aerobic (45°C) and aerobic (55°C) conditions respectively. The bioreactor operated at 55°C presented a higher mineralization rate compared to 35°C throughout the experimental period, suggesting accelerated biodegradation rates under thermophilic condition. A lag phase in microbial mineralization was observed at 35°C, which was even prolonged at 45°C. In addition, ammonia was significantly stripped out due to aeration. DNA was extracted from leachate sample after one month of bioreactor operation, in order to reveal the microbial variation during the start-up stage. Analysis on the 16s rRNA gene showed that the diversity of the bacterial community was increased after aeration. The Proteobacteria was identified as the most dominant group under the thermophilic temperature. They may exhibit a positive impact on the aerobic bioconversion, as many members of this phylum have diverse metabolic capabilities. Bachelor of Engineering (Environmental Engineering) 2015-05-25T07:09:01Z 2015-05-25T07:09:01Z 2015 2015 Final Year Project (FYP) http://hdl.handle.net/10356/64236 en Nanyang Technological University 65 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Environmental engineering::Waste management
spellingShingle DRNTU::Engineering::Environmental engineering::Waste management
Ng, Wei Hong
Investigation on the microbial profile and waste stabilization during in situ aeration of landfill bioreactors at different temperature
description In situ aeration has been a popular and promising method for the advantages it bring about such as reduction of emission of greenhouse gases, accelerating of waste stabilization and settlement, decrease in concentrations of leachate contaminants and being able to provide the optimal conditions for microbial decomposition. The purpose of this study is to investigate and develop a better understanding of the influences of different temperature on the aeration performances and microbial communities. Hence, this give rises to our study using four Landfill Bioreactors (LBRs) NR35 (control), AR35, AR45 and AR55 under anaerobic (35°C), aerobic (35°C), aerobic (45°C) and aerobic (55°C) conditions respectively. The bioreactor operated at 55°C presented a higher mineralization rate compared to 35°C throughout the experimental period, suggesting accelerated biodegradation rates under thermophilic condition. A lag phase in microbial mineralization was observed at 35°C, which was even prolonged at 45°C. In addition, ammonia was significantly stripped out due to aeration. DNA was extracted from leachate sample after one month of bioreactor operation, in order to reveal the microbial variation during the start-up stage. Analysis on the 16s rRNA gene showed that the diversity of the bacterial community was increased after aeration. The Proteobacteria was identified as the most dominant group under the thermophilic temperature. They may exhibit a positive impact on the aerobic bioconversion, as many members of this phylum have diverse metabolic capabilities.
author2 Wang Jing-Yuan
author_facet Wang Jing-Yuan
Ng, Wei Hong
format Final Year Project
author Ng, Wei Hong
author_sort Ng, Wei Hong
title Investigation on the microbial profile and waste stabilization during in situ aeration of landfill bioreactors at different temperature
title_short Investigation on the microbial profile and waste stabilization during in situ aeration of landfill bioreactors at different temperature
title_full Investigation on the microbial profile and waste stabilization during in situ aeration of landfill bioreactors at different temperature
title_fullStr Investigation on the microbial profile and waste stabilization during in situ aeration of landfill bioreactors at different temperature
title_full_unstemmed Investigation on the microbial profile and waste stabilization during in situ aeration of landfill bioreactors at different temperature
title_sort investigation on the microbial profile and waste stabilization during in situ aeration of landfill bioreactors at different temperature
publishDate 2015
url http://hdl.handle.net/10356/64236
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