Assembled AU@PDA nanocrystals on reduced graphene oxide modified glassy carbon electrode for detection of multiplex DNA

We report here a successful multiplex DNA targets biosensor using reduced graphene oxide modified glassy carbon electrode (rGO-GCE). On the surface, a monolay of 50 nm Au@PDA nanocrystals were decorated on it. The modified Hummer’s method was used to chemically synthesize Graphene oxide (GO) and the...

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Main Author: Ng, Serene Shi Ya
Other Authors: Duan Hongwei
Format: Final Year Project
Language:English
Published: 2015
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Online Access:http://hdl.handle.net/10356/64979
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-649792023-03-03T15:38:02Z Assembled AU@PDA nanocrystals on reduced graphene oxide modified glassy carbon electrode for detection of multiplex DNA Ng, Serene Shi Ya Duan Hongwei School of Chemical and Biomedical Engineering DRNTU::Engineering::Bioengineering We report here a successful multiplex DNA targets biosensor using reduced graphene oxide modified glassy carbon electrode (rGO-GCE). On the surface, a monolay of 50 nm Au@PDA nanocrystals were decorated on it. The modified Hummer’s method was used to chemically synthesize Graphene oxide (GO) and then electrochemically reduce to reduced graphene oxide (rGO). The rGO-GCE was obtained by reducing electrochemically in 0.1 M phosphate buffer solution at pH 7.0 via voltammetry cycling (2 cycles). Inspired by the composition of adhesive proteins in mussels, polydopamine film was formed by self-polymerizing dopamine and then adhered to the gold nanoparticles (Au NPs). Quantum dots (QDs) of metal sulfide nanocrystals (ZnS, CdS, PbS, CuS) were used as detection variable by dissolving the QDs which generates electrochemical signals that indicates the concentration of the DNA targets. The tests were carried out at the best condition of supporting electrolyte of sodium acetate (NaAc) at the concentration of 0.10 M and at the pH of 6.0. Multiplex DNA targets detection was successfully carried out with a linear range for DNA targets T1 (p16), T2 (p21), T3 (p53), and T4 (p73) ranging from 5 nM to 1 µM, 1 nM to 1 µM, 300 pM to 100 nM and 10 nM to 10 µM, with a detection limit of 2 nM, 300 pM, 100 pM and 3 nM, correspondingly. The study showed that even in the multiplex DNA detection, the electrode still displayed excellent selectivity and reproducibility. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2015-06-10T03:00:52Z 2015-06-10T03:00:52Z 2015 2015 Final Year Project (FYP) http://hdl.handle.net/10356/64979 en Nanyang Technological University 40 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Bioengineering
spellingShingle DRNTU::Engineering::Bioengineering
Ng, Serene Shi Ya
Assembled AU@PDA nanocrystals on reduced graphene oxide modified glassy carbon electrode for detection of multiplex DNA
description We report here a successful multiplex DNA targets biosensor using reduced graphene oxide modified glassy carbon electrode (rGO-GCE). On the surface, a monolay of 50 nm Au@PDA nanocrystals were decorated on it. The modified Hummer’s method was used to chemically synthesize Graphene oxide (GO) and then electrochemically reduce to reduced graphene oxide (rGO). The rGO-GCE was obtained by reducing electrochemically in 0.1 M phosphate buffer solution at pH 7.0 via voltammetry cycling (2 cycles). Inspired by the composition of adhesive proteins in mussels, polydopamine film was formed by self-polymerizing dopamine and then adhered to the gold nanoparticles (Au NPs). Quantum dots (QDs) of metal sulfide nanocrystals (ZnS, CdS, PbS, CuS) were used as detection variable by dissolving the QDs which generates electrochemical signals that indicates the concentration of the DNA targets. The tests were carried out at the best condition of supporting electrolyte of sodium acetate (NaAc) at the concentration of 0.10 M and at the pH of 6.0. Multiplex DNA targets detection was successfully carried out with a linear range for DNA targets T1 (p16), T2 (p21), T3 (p53), and T4 (p73) ranging from 5 nM to 1 µM, 1 nM to 1 µM, 300 pM to 100 nM and 10 nM to 10 µM, with a detection limit of 2 nM, 300 pM, 100 pM and 3 nM, correspondingly. The study showed that even in the multiplex DNA detection, the electrode still displayed excellent selectivity and reproducibility.
author2 Duan Hongwei
author_facet Duan Hongwei
Ng, Serene Shi Ya
format Final Year Project
author Ng, Serene Shi Ya
author_sort Ng, Serene Shi Ya
title Assembled AU@PDA nanocrystals on reduced graphene oxide modified glassy carbon electrode for detection of multiplex DNA
title_short Assembled AU@PDA nanocrystals on reduced graphene oxide modified glassy carbon electrode for detection of multiplex DNA
title_full Assembled AU@PDA nanocrystals on reduced graphene oxide modified glassy carbon electrode for detection of multiplex DNA
title_fullStr Assembled AU@PDA nanocrystals on reduced graphene oxide modified glassy carbon electrode for detection of multiplex DNA
title_full_unstemmed Assembled AU@PDA nanocrystals on reduced graphene oxide modified glassy carbon electrode for detection of multiplex DNA
title_sort assembled au@pda nanocrystals on reduced graphene oxide modified glassy carbon electrode for detection of multiplex dna
publishDate 2015
url http://hdl.handle.net/10356/64979
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