Viability nanosensors to evaluate cell interaction with novel nanomaterials

The engineering of cells using nanoparticles is a promising approach for integration free labelled cells as well as to determine the viability of the implanted cells for treatments. Particularly in the medical field, it is essential to understand the properties of nanoparticles and any possible effe...

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Main Author: Goh, Candice Yun Si
Other Authors: Xu Chenjie
Format: Final Year Project
Language:English
Published: 2015
Subjects:
Online Access:http://hdl.handle.net/10356/65062
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-650622023-03-03T15:32:04Z Viability nanosensors to evaluate cell interaction with novel nanomaterials Goh, Candice Yun Si Xu Chenjie School of Chemical and Biomedical Engineering DRNTU::Engineering::Bioengineering The engineering of cells using nanoparticles is a promising approach for integration free labelled cells as well as to determine the viability of the implanted cells for treatments. Particularly in the medical field, it is essential to understand the properties of nanoparticles and any possible effects on the human body before clinical use. Despite the potential nanoparticles display, limited researches are done on the toxicity of nanoparticles and little is known about the toxicity of different nanoparticles due to varying experimental controls in the studies that have been done. Therefore, it is difficult to determining whether the cytotoxicity observed is physiologically relevant. In this study, characterization of calcein-AM loaded PLGA microparticles coated with poly-l-lysine illustrates enhanced intracellular internalization. Furthermore, cytotoxicity of various and novel nanoparticles are investigated to determine an easy and safe method to quantify the viability of nanosensors. Thereafter, in vitro experiment of novel nanoparticles demonstrated that they can non-invasively determine cell viability. Hence, the study of cytotoxicity of various and novel nanoparticles is useful for identifying safe and easy methods to engineer cells for therapeutic treatments. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2015-06-11T07:00:45Z 2015-06-11T07:00:45Z 2015 2015 Final Year Project (FYP) http://hdl.handle.net/10356/65062 en Nanyang Technological University 49 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Bioengineering
spellingShingle DRNTU::Engineering::Bioengineering
Goh, Candice Yun Si
Viability nanosensors to evaluate cell interaction with novel nanomaterials
description The engineering of cells using nanoparticles is a promising approach for integration free labelled cells as well as to determine the viability of the implanted cells for treatments. Particularly in the medical field, it is essential to understand the properties of nanoparticles and any possible effects on the human body before clinical use. Despite the potential nanoparticles display, limited researches are done on the toxicity of nanoparticles and little is known about the toxicity of different nanoparticles due to varying experimental controls in the studies that have been done. Therefore, it is difficult to determining whether the cytotoxicity observed is physiologically relevant. In this study, characterization of calcein-AM loaded PLGA microparticles coated with poly-l-lysine illustrates enhanced intracellular internalization. Furthermore, cytotoxicity of various and novel nanoparticles are investigated to determine an easy and safe method to quantify the viability of nanosensors. Thereafter, in vitro experiment of novel nanoparticles demonstrated that they can non-invasively determine cell viability. Hence, the study of cytotoxicity of various and novel nanoparticles is useful for identifying safe and easy methods to engineer cells for therapeutic treatments.
author2 Xu Chenjie
author_facet Xu Chenjie
Goh, Candice Yun Si
format Final Year Project
author Goh, Candice Yun Si
author_sort Goh, Candice Yun Si
title Viability nanosensors to evaluate cell interaction with novel nanomaterials
title_short Viability nanosensors to evaluate cell interaction with novel nanomaterials
title_full Viability nanosensors to evaluate cell interaction with novel nanomaterials
title_fullStr Viability nanosensors to evaluate cell interaction with novel nanomaterials
title_full_unstemmed Viability nanosensors to evaluate cell interaction with novel nanomaterials
title_sort viability nanosensors to evaluate cell interaction with novel nanomaterials
publishDate 2015
url http://hdl.handle.net/10356/65062
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