In vitro study of liver tumor induced endothelial cell angiogenesis in microfluidic platform
Cancer cells metastasis is an important medical problem which causes 90% of human cancer deaths. Angiogenesis plays essential roles in metastasis and secondary tumor growth. Extensive study on tumor angiogenesis is required to understand the mechanism of tumor angiogenesis and to find out efficient...
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sg-ntu-dr.10356-650852023-03-03T15:35:23Z In vitro study of liver tumor induced endothelial cell angiogenesis in microfluidic platform Xie, Yufei Kathy, Qian Luo School of Chemical and Biomedical Engineering DRNTU::Engineering::Bioengineering Cancer cells metastasis is an important medical problem which causes 90% of human cancer deaths. Angiogenesis plays essential roles in metastasis and secondary tumor growth. Extensive study on tumor angiogenesis is required to understand the mechanism of tumor angiogenesis and to find out efficient anti-angiogenic therapy. Microfluidic system, mimicking the real metastatic physiological situations, is increasingly being utilized to study tumor angiogenesis. In this study, a new 3D microfluidic model was designed and applied to study the effect of liver tumor-induced endothelial angiogenesis. Human umbilical vein endothelial cells (HUVEC-C3) transfected with the caspase-3-based FRET biosensor that are capable of indicating caspase-3 activation were seeded into microchannels, and results indicated that the addition of liver hepatocellular carcinoma (Hep-G2) cell can promote the angiogenesis of HUVEC-C3 cells towards the Hep-G2 cell spheroids. Subsequently, the successfully designed 3D micro-fluidic systems will be utilized to study the effect of angiogenesis inducing factors vessel-endothelial growth factor (VEGF) and tumor growth factor-beta (TGF-beta), anti-cancer drug (Taxol and angiogenesis inhibitor as well. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2015-06-15T01:22:45Z 2015-06-15T01:22:45Z 2015 2015 Final Year Project (FYP) http://hdl.handle.net/10356/65085 en Nanyang Technological University 59 p. application/pdf |
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DRNTU::Engineering::Bioengineering Xie, Yufei In vitro study of liver tumor induced endothelial cell angiogenesis in microfluidic platform |
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Cancer cells metastasis is an important medical problem which causes 90% of human cancer deaths. Angiogenesis plays essential roles in metastasis and secondary tumor growth. Extensive study on tumor angiogenesis is required to understand the mechanism of tumor angiogenesis and to find out efficient anti-angiogenic therapy. Microfluidic system, mimicking the real metastatic physiological situations, is increasingly being utilized to study tumor angiogenesis. In this study, a new 3D microfluidic model was designed and applied to study the effect of liver tumor-induced endothelial angiogenesis. Human umbilical vein endothelial cells (HUVEC-C3) transfected with the caspase-3-based FRET biosensor that are capable of indicating caspase-3 activation were seeded into microchannels, and results indicated that the addition of liver hepatocellular carcinoma (Hep-G2) cell can promote the angiogenesis of HUVEC-C3 cells towards the Hep-G2 cell spheroids. Subsequently, the successfully designed 3D micro-fluidic systems will be utilized to study the effect of angiogenesis inducing factors vessel-endothelial growth factor (VEGF) and tumor growth factor-beta (TGF-beta), anti-cancer drug (Taxol and angiogenesis inhibitor as well. |
author2 |
Kathy, Qian Luo |
author_facet |
Kathy, Qian Luo Xie, Yufei |
format |
Final Year Project |
author |
Xie, Yufei |
author_sort |
Xie, Yufei |
title |
In vitro study of liver tumor induced endothelial cell angiogenesis in microfluidic platform |
title_short |
In vitro study of liver tumor induced endothelial cell angiogenesis in microfluidic platform |
title_full |
In vitro study of liver tumor induced endothelial cell angiogenesis in microfluidic platform |
title_fullStr |
In vitro study of liver tumor induced endothelial cell angiogenesis in microfluidic platform |
title_full_unstemmed |
In vitro study of liver tumor induced endothelial cell angiogenesis in microfluidic platform |
title_sort |
in vitro study of liver tumor induced endothelial cell angiogenesis in microfluidic platform |
publishDate |
2015 |
url |
http://hdl.handle.net/10356/65085 |
_version_ |
1759855037560913920 |