Cyclic compression of mesenchymal stem cells loaded scaffolds for bone tissue engineering

Cyclic mechanical loading on Mesenchymal Stem Cells (MSCs) has resulted in increased expression of early osteogenic markers as well as increased mineralized matrix deposition. In most of past studies, scaffolds were subjected to the cyclic loading immediately after seeding or a few days after static...

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Main Author: Guo, Fangfang
Other Authors: Teoh Swee Hin
Format: Final Year Project
Language:English
Published: 2015
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Online Access:http://hdl.handle.net/10356/65101
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-651012023-03-03T15:40:26Z Cyclic compression of mesenchymal stem cells loaded scaffolds for bone tissue engineering Guo, Fangfang Teoh Swee Hin School of Chemical and Biomedical Engineering DRNTU::Engineering::Bioengineering Cyclic mechanical loading on Mesenchymal Stem Cells (MSCs) has resulted in increased expression of early osteogenic markers as well as increased mineralized matrix deposition. In most of past studies, scaffolds were subjected to the cyclic loading immediately after seeding or a few days after static culture. However, sufficient cell numbers and stable attachment of cells could enhance better mechanotransduction and accelerate bone formation. The aim of the study was to investigate the osteogenic responses of well proliferated human fetal mesenchymal stem cells (hfMSC) seeded scaffolds to cyclic compression loading, using biaxial rotating bioreactor to proliferate the cells first and the mini static bioreactor to induce appropriate load and strain. Preliminary study was done to investigate the most effective strain that enhances bone formation of hfMSCs. Preliminary study demonstrated that cyclic compression at physiological related strain (2,200 μɛ) best enhances osteogenesis and proliferation of hfMSCs, and this strain was applied in the second study. Results in the second study showed that proliferation was inhibited in the loaded group in a short-term culture (7 days) while enhanced in long-term (14 days). ALP activity was enhanced (1.35 fold) in cyclic loaded group at day 14, which in turn result in promoted mineralization. Conclusion can be drawn that cyclic compression enhances the osteogenesis and mineralization on well proliferated cellular scaffolds. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2015-06-15T02:01:25Z 2015-06-15T02:01:25Z 2015 2015 Final Year Project (FYP) http://hdl.handle.net/10356/65101 en Nanyang Technological University 56 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Bioengineering
spellingShingle DRNTU::Engineering::Bioengineering
Guo, Fangfang
Cyclic compression of mesenchymal stem cells loaded scaffolds for bone tissue engineering
description Cyclic mechanical loading on Mesenchymal Stem Cells (MSCs) has resulted in increased expression of early osteogenic markers as well as increased mineralized matrix deposition. In most of past studies, scaffolds were subjected to the cyclic loading immediately after seeding or a few days after static culture. However, sufficient cell numbers and stable attachment of cells could enhance better mechanotransduction and accelerate bone formation. The aim of the study was to investigate the osteogenic responses of well proliferated human fetal mesenchymal stem cells (hfMSC) seeded scaffolds to cyclic compression loading, using biaxial rotating bioreactor to proliferate the cells first and the mini static bioreactor to induce appropriate load and strain. Preliminary study was done to investigate the most effective strain that enhances bone formation of hfMSCs. Preliminary study demonstrated that cyclic compression at physiological related strain (2,200 μɛ) best enhances osteogenesis and proliferation of hfMSCs, and this strain was applied in the second study. Results in the second study showed that proliferation was inhibited in the loaded group in a short-term culture (7 days) while enhanced in long-term (14 days). ALP activity was enhanced (1.35 fold) in cyclic loaded group at day 14, which in turn result in promoted mineralization. Conclusion can be drawn that cyclic compression enhances the osteogenesis and mineralization on well proliferated cellular scaffolds.
author2 Teoh Swee Hin
author_facet Teoh Swee Hin
Guo, Fangfang
format Final Year Project
author Guo, Fangfang
author_sort Guo, Fangfang
title Cyclic compression of mesenchymal stem cells loaded scaffolds for bone tissue engineering
title_short Cyclic compression of mesenchymal stem cells loaded scaffolds for bone tissue engineering
title_full Cyclic compression of mesenchymal stem cells loaded scaffolds for bone tissue engineering
title_fullStr Cyclic compression of mesenchymal stem cells loaded scaffolds for bone tissue engineering
title_full_unstemmed Cyclic compression of mesenchymal stem cells loaded scaffolds for bone tissue engineering
title_sort cyclic compression of mesenchymal stem cells loaded scaffolds for bone tissue engineering
publishDate 2015
url http://hdl.handle.net/10356/65101
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