Investigating the toxicity of ATA nanoparticles in vitro and cardioprotective effects of ATA on DOX-induced cardiotoxicity in zebrafish

A novel compound acetyltanshinone (ATA) has been discovered to have a prominent growth inhibition effect on breast cancer cells and may be potentially used as a chemotherapeutic drug. However, its property of low aqueous solubility and bioavailability poses a limitation on its drug efficacy. Therefo...

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Main Author: Lee, Eden Hui Ling
Other Authors: Luo Qian Kathy
Format: Final Year Project
Language:English
Published: 2015
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Online Access:http://hdl.handle.net/10356/65228
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-652282023-03-03T15:40:10Z Investigating the toxicity of ATA nanoparticles in vitro and cardioprotective effects of ATA on DOX-induced cardiotoxicity in zebrafish Lee, Eden Hui Ling Luo Qian Kathy School of Chemical and Biomedical Engineering DRNTU::Engineering::Bioengineering A novel compound acetyltanshinone (ATA) has been discovered to have a prominent growth inhibition effect on breast cancer cells and may be potentially used as a chemotherapeutic drug. However, its property of low aqueous solubility and bioavailability poses a limitation on its drug efficacy. Therefore, nanoparticle drug loading, a method that is able to increase the drug’s bioavailability, was performed. The key objective of this final year project was to investigate the in vitro and in vivo effects of ATA nanoparticles. Based on the MTT assay, the ATA nanoparticles were found to have comparable drug efficacy as ATA in free form where the cell inhibition rate on cancer cells were in the range of 80-90% after 72 hours of treatment. Toxicity screening of free ATA and ATA nanoparticles in the zebrafish model was also carried out and the ATA nanoparticles were found to reduce the toxic effects of free ATA. Higher concentrations of free ATA at 20 μM and 40 μM were found to cause developmental malformations and mortality in zebrafish. A further study on whether ATA possessed cardioprotective effects against doxorubicin (DOX)-induced cardiac toxicity was carried out. Safe concentrations of free ATA were used to treat zebrafish embryos together with the usage of 50 μM of DOX. The results revealed the use of 5 μM and 10 μM was able to alleviate the DOX-induced cardiac toxicity as shown from lower incidences of cardiac abnormalities and higher heart rates compared to the group treated with solely DOX. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2015-06-17T02:27:49Z 2015-06-17T02:27:49Z 2015 2015 Final Year Project (FYP) http://hdl.handle.net/10356/65228 en Nanyang Technological University 58 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Bioengineering
spellingShingle DRNTU::Engineering::Bioengineering
Lee, Eden Hui Ling
Investigating the toxicity of ATA nanoparticles in vitro and cardioprotective effects of ATA on DOX-induced cardiotoxicity in zebrafish
description A novel compound acetyltanshinone (ATA) has been discovered to have a prominent growth inhibition effect on breast cancer cells and may be potentially used as a chemotherapeutic drug. However, its property of low aqueous solubility and bioavailability poses a limitation on its drug efficacy. Therefore, nanoparticle drug loading, a method that is able to increase the drug’s bioavailability, was performed. The key objective of this final year project was to investigate the in vitro and in vivo effects of ATA nanoparticles. Based on the MTT assay, the ATA nanoparticles were found to have comparable drug efficacy as ATA in free form where the cell inhibition rate on cancer cells were in the range of 80-90% after 72 hours of treatment. Toxicity screening of free ATA and ATA nanoparticles in the zebrafish model was also carried out and the ATA nanoparticles were found to reduce the toxic effects of free ATA. Higher concentrations of free ATA at 20 μM and 40 μM were found to cause developmental malformations and mortality in zebrafish. A further study on whether ATA possessed cardioprotective effects against doxorubicin (DOX)-induced cardiac toxicity was carried out. Safe concentrations of free ATA were used to treat zebrafish embryos together with the usage of 50 μM of DOX. The results revealed the use of 5 μM and 10 μM was able to alleviate the DOX-induced cardiac toxicity as shown from lower incidences of cardiac abnormalities and higher heart rates compared to the group treated with solely DOX.
author2 Luo Qian Kathy
author_facet Luo Qian Kathy
Lee, Eden Hui Ling
format Final Year Project
author Lee, Eden Hui Ling
author_sort Lee, Eden Hui Ling
title Investigating the toxicity of ATA nanoparticles in vitro and cardioprotective effects of ATA on DOX-induced cardiotoxicity in zebrafish
title_short Investigating the toxicity of ATA nanoparticles in vitro and cardioprotective effects of ATA on DOX-induced cardiotoxicity in zebrafish
title_full Investigating the toxicity of ATA nanoparticles in vitro and cardioprotective effects of ATA on DOX-induced cardiotoxicity in zebrafish
title_fullStr Investigating the toxicity of ATA nanoparticles in vitro and cardioprotective effects of ATA on DOX-induced cardiotoxicity in zebrafish
title_full_unstemmed Investigating the toxicity of ATA nanoparticles in vitro and cardioprotective effects of ATA on DOX-induced cardiotoxicity in zebrafish
title_sort investigating the toxicity of ata nanoparticles in vitro and cardioprotective effects of ata on dox-induced cardiotoxicity in zebrafish
publishDate 2015
url http://hdl.handle.net/10356/65228
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