Molecular dissection of the assembly and morphogenesis processes of corona virus- the essential roles of the viral envelope protein.

To promote viral entry, replication, release and spread to neighboring cells, many cytolytic animal viruses encode proteins responsible for modification of host cell membrane permeability and for formation of ion channels in host cell membranes during their life cycles. In this study, we show that t...

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Main Author: Liu, Ding Xiang.
Other Authors: Liu Ding Xiang
Format: Research Report
Published: 2008
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Online Access:http://hdl.handle.net/10356/6561
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-65612023-02-28T17:58:14Z Molecular dissection of the assembly and morphogenesis processes of corona virus- the essential roles of the viral envelope protein. Liu, Ding Xiang. Liu Ding Xiang School of Biological Sciences DRNTU::Science::Biological sciences To promote viral entry, replication, release and spread to neighboring cells, many cytolytic animal viruses encode proteins responsible for modification of host cell membrane permeability and for formation of ion channels in host cell membranes during their life cycles. In this study, we show that the envelope (E) protein of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) can induce membrane permeability changes when expressed in E. coli. E protein expressed in bacterial and mammalian cells under reducing conditions existed as monomers, but formed homodimer and homotrimer under non-reducing conditions. Site-directed mutagenesis studies revealed that two cysteine residues of the E protein were essential for oligomerization, leading to induction of membrane permeability. This is the first report demonstrating that a coronavirus-encoded protein could modify membrane permeability. 2008-09-17T11:40:06Z 2008-09-17T11:40:06Z 2003 2003 Research Report http://hdl.handle.net/10356/6561 Nanyang Technological University application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Liu, Ding Xiang.
Molecular dissection of the assembly and morphogenesis processes of corona virus- the essential roles of the viral envelope protein.
description To promote viral entry, replication, release and spread to neighboring cells, many cytolytic animal viruses encode proteins responsible for modification of host cell membrane permeability and for formation of ion channels in host cell membranes during their life cycles. In this study, we show that the envelope (E) protein of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) can induce membrane permeability changes when expressed in E. coli. E protein expressed in bacterial and mammalian cells under reducing conditions existed as monomers, but formed homodimer and homotrimer under non-reducing conditions. Site-directed mutagenesis studies revealed that two cysteine residues of the E protein were essential for oligomerization, leading to induction of membrane permeability. This is the first report demonstrating that a coronavirus-encoded protein could modify membrane permeability.
author2 Liu Ding Xiang
author_facet Liu Ding Xiang
Liu, Ding Xiang.
format Research Report
author Liu, Ding Xiang.
author_sort Liu, Ding Xiang.
title Molecular dissection of the assembly and morphogenesis processes of corona virus- the essential roles of the viral envelope protein.
title_short Molecular dissection of the assembly and morphogenesis processes of corona virus- the essential roles of the viral envelope protein.
title_full Molecular dissection of the assembly and morphogenesis processes of corona virus- the essential roles of the viral envelope protein.
title_fullStr Molecular dissection of the assembly and morphogenesis processes of corona virus- the essential roles of the viral envelope protein.
title_full_unstemmed Molecular dissection of the assembly and morphogenesis processes of corona virus- the essential roles of the viral envelope protein.
title_sort molecular dissection of the assembly and morphogenesis processes of corona virus- the essential roles of the viral envelope protein.
publishDate 2008
url http://hdl.handle.net/10356/6561
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