Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni
Several environmental pollutants, including biodegradable and non-biodegradable pollutants, under conditions of high enough concentrations can cause serious damage to the living organisms. Chloroanilines as nitrogen-containing aromatic compounds and one of xenobiotic pollutants are widely used chemi...
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sg-ntu-dr.10356-657682023-03-03T17:10:55Z Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni Qian, Duoduo Cao Bin School of Civil and Environmental Engineering Singapore Centre for Environmental Life Sciences Engineering DRNTU::Engineering::Environmental engineering Several environmental pollutants, including biodegradable and non-biodegradable pollutants, under conditions of high enough concentrations can cause serious damage to the living organisms. Chloroanilines as nitrogen-containing aromatic compounds and one of xenobiotic pollutants are widely used chemicals in industry for the production of polyurethanes, rubber, azo dyes, drugs, and pesticides, in which 3-Chloroaniline is proved to have an inhibitive effect on bacterial growth. Comamonas testosteroni WDL7 were proved to have the ability of degrading 3-CA. C-di-GMP is a secondary messenger in bacteria determines bacterial life between the planktonic and biofilm life modes, which is synthesized by GGDEF domain proteins and degraded by EAL domain proteins. YedQ is a GGDEF domain protein and yhjH is of EAL domain, which can be mutated with pyedQ and pyhjH induction. Comamonas testosteroni WDL7 wild type, yedQ, and yhjH mutants were incubated with 3-cholroanilne as the sole carbon source in planktonic environment, in which mutant yedQ showed a better performance on growth and surviving, pyhjH mutant showed a delayed degradation profile. Bachelor of Engineering (Environmental Engineering) 2015-12-14T03:23:58Z 2015-12-14T03:23:58Z 2015 2015 Final Year Project (FYP) http://hdl.handle.net/10356/65768 en Nanyang Technological University 35 p. application/pdf |
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DRNTU::Engineering::Environmental engineering Qian, Duoduo Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni |
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Several environmental pollutants, including biodegradable and non-biodegradable pollutants, under conditions of high enough concentrations can cause serious damage to the living organisms. Chloroanilines as nitrogen-containing aromatic compounds and one of xenobiotic pollutants are widely used chemicals in industry for the production of polyurethanes, rubber, azo dyes, drugs, and pesticides, in which 3-Chloroaniline is proved to have an inhibitive effect on bacterial growth. Comamonas testosteroni WDL7 were proved to have the ability of degrading 3-CA. C-di-GMP is a secondary messenger in bacteria determines bacterial life between the planktonic and biofilm life modes, which is synthesized by GGDEF domain proteins and degraded by EAL domain proteins. YedQ is a GGDEF domain protein and yhjH is of EAL domain, which can be mutated with pyedQ and pyhjH induction. Comamonas testosteroni WDL7 wild type, yedQ, and yhjH mutants were incubated with 3-cholroanilne as the sole carbon source in planktonic environment, in which mutant yedQ showed a better performance on growth and surviving, pyhjH mutant showed a delayed degradation profile. |
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Cao Bin |
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Cao Bin Qian, Duoduo |
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Final Year Project |
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Qian, Duoduo |
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Qian, Duoduo |
title |
Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni |
title_short |
Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni |
title_full |
Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni |
title_fullStr |
Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni |
title_full_unstemmed |
Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni |
title_sort |
preliminary study on the effect of high and low cyclic-di-gmp level on 3-chloroaniline biodegradation by comamonas testosteroni |
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2015 |
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http://hdl.handle.net/10356/65768 |
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1759858290410389504 |