Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni

Several environmental pollutants, including biodegradable and non-biodegradable pollutants, under conditions of high enough concentrations can cause serious damage to the living organisms. Chloroanilines as nitrogen-containing aromatic compounds and one of xenobiotic pollutants are widely used chemi...

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Main Author: Qian, Duoduo
Other Authors: Cao Bin
Format: Final Year Project
Language:English
Published: 2015
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Online Access:http://hdl.handle.net/10356/65768
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-657682023-03-03T17:10:55Z Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni Qian, Duoduo Cao Bin School of Civil and Environmental Engineering Singapore Centre for Environmental Life Sciences Engineering DRNTU::Engineering::Environmental engineering Several environmental pollutants, including biodegradable and non-biodegradable pollutants, under conditions of high enough concentrations can cause serious damage to the living organisms. Chloroanilines as nitrogen-containing aromatic compounds and one of xenobiotic pollutants are widely used chemicals in industry for the production of polyurethanes, rubber, azo dyes, drugs, and pesticides, in which 3-Chloroaniline is proved to have an inhibitive effect on bacterial growth. Comamonas testosteroni WDL7 were proved to have the ability of degrading 3-CA. C-di-GMP is a secondary messenger in bacteria determines bacterial life between the planktonic and biofilm life modes, which is synthesized by GGDEF domain proteins and degraded by EAL domain proteins. YedQ is a GGDEF domain protein and yhjH is of EAL domain, which can be mutated with pyedQ and pyhjH induction. Comamonas testosteroni WDL7 wild type, yedQ, and yhjH mutants were incubated with 3-cholroanilne as the sole carbon source in planktonic environment, in which mutant yedQ showed a better performance on growth and surviving, pyhjH mutant showed a delayed degradation profile. Bachelor of Engineering (Environmental Engineering) 2015-12-14T03:23:58Z 2015-12-14T03:23:58Z 2015 2015 Final Year Project (FYP) http://hdl.handle.net/10356/65768 en Nanyang Technological University 35 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Environmental engineering
spellingShingle DRNTU::Engineering::Environmental engineering
Qian, Duoduo
Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni
description Several environmental pollutants, including biodegradable and non-biodegradable pollutants, under conditions of high enough concentrations can cause serious damage to the living organisms. Chloroanilines as nitrogen-containing aromatic compounds and one of xenobiotic pollutants are widely used chemicals in industry for the production of polyurethanes, rubber, azo dyes, drugs, and pesticides, in which 3-Chloroaniline is proved to have an inhibitive effect on bacterial growth. Comamonas testosteroni WDL7 were proved to have the ability of degrading 3-CA. C-di-GMP is a secondary messenger in bacteria determines bacterial life between the planktonic and biofilm life modes, which is synthesized by GGDEF domain proteins and degraded by EAL domain proteins. YedQ is a GGDEF domain protein and yhjH is of EAL domain, which can be mutated with pyedQ and pyhjH induction. Comamonas testosteroni WDL7 wild type, yedQ, and yhjH mutants were incubated with 3-cholroanilne as the sole carbon source in planktonic environment, in which mutant yedQ showed a better performance on growth and surviving, pyhjH mutant showed a delayed degradation profile.
author2 Cao Bin
author_facet Cao Bin
Qian, Duoduo
format Final Year Project
author Qian, Duoduo
author_sort Qian, Duoduo
title Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni
title_short Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni
title_full Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni
title_fullStr Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni
title_full_unstemmed Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni
title_sort preliminary study on the effect of high and low cyclic-di-gmp level on 3-chloroaniline biodegradation by comamonas testosteroni
publishDate 2015
url http://hdl.handle.net/10356/65768
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