Osmogen mediated hollow microparticles fabrication for peptide encapsulation and delivery

• Main Author: Lee, Xuan Yi
• Other Authors: Loo Say Chye Joachim
• Format: Final Year Project
• Language: English
• Published: 2016
• Subjects: DRNTU::Engineering
• Online Access: http://hdl.handle.net/10356/66345
• Institution: Nanyang Technological University

Bioactive molecules such as peptides and proteins have significant therapeutic potential. However, their therapeutic ability is limited by their low systemic stability, short half-life, and subsequent loss of bioactivity. In order to increase their stability, drug efficacy, and to achieve a sustained release profile, encapsulation with poly(lactic-co-glycolic acid) (PLGA), is carried out. PLGA hollow microparticles were fabricated by a novel osmogen mediated solvent emulsion evaporation technique with the aim of minimizing the creation of a localized acidic microenvironment, which causes peptide denaturation. The effects of various process parameters on the morphology of the hollow microparticles were studied and characterized by scanning electron microscope (SEM). Thereafter, these hollow microparticles were loaded with the peptide, Glucagon-like peptide-1 (GLP-1), and its subsequent encapsulation efficiency and in vitro release behaviour were analyzed.