Investigating the relationship between marine and air microbiome in Singapore

Air-sea transfer is crucial for microbial dispersal and maintenance of diversity in the marine environments. Air may be viewed as a medium of transfer necessary for microorganisms to reproduce and disperse to new sites when the old site becomes overpopulated and nutrient-limiting. It is hypothesised...

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Main Author: Lau, Kenny Jia Xu
Other Authors: Enzo Acerbi
Format: Final Year Project
Language:English
Published: 2016
Subjects:
Online Access:http://hdl.handle.net/10356/66996
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-669962023-02-28T17:59:39Z Investigating the relationship between marine and air microbiome in Singapore Lau, Kenny Jia Xu Enzo Acerbi Federico Lauro School of Biological Sciences Singapore Centre for Environmental Life Sciences Engineering DRNTU::Science::Biological sciences::Ecology Air-sea transfer is crucial for microbial dispersal and maintenance of diversity in the marine environments. Air may be viewed as a medium of transfer necessary for microorganisms to reproduce and disperse to new sites when the old site becomes overpopulated and nutrient-limiting. It is hypothesised that microorganisms are constantly aerosolised from surface seawater into the air by splashing water and wind. Nonetheless, the air is a hostile environment to live in due to abiotic stresses like desiccation and ultraviolet radiation. In this study, metagenomics sequencing was used to investigate the air-sea transfer of microorganisms from DNA isolated from marine and air environments. During Ocean Sampling Day (OSD) on 21 June 2015, samples were collected from different coastal sites around Singapore. The samples were sequenced and a set of common species was identified across marine and air samples. They include bacteria species such as Gemmatimonadetes bacterium, Gemmata obscuriglobus and Actinobacterium acAcidi, and fungal species, Rhizophagus irregularis, which are reported in previous studies to have abiotic stress adaptations. In addition, this study will also provide a basis for improvements and optimisation of sampling protocols for OSD 2016. Bachelor of Science in Biological Sciences 2016-05-10T01:47:29Z 2016-05-10T01:47:29Z 2016 Final Year Project (FYP) http://hdl.handle.net/10356/66996 en Nanyang Technological University 40 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Ecology
spellingShingle DRNTU::Science::Biological sciences::Ecology
Lau, Kenny Jia Xu
Investigating the relationship between marine and air microbiome in Singapore
description Air-sea transfer is crucial for microbial dispersal and maintenance of diversity in the marine environments. Air may be viewed as a medium of transfer necessary for microorganisms to reproduce and disperse to new sites when the old site becomes overpopulated and nutrient-limiting. It is hypothesised that microorganisms are constantly aerosolised from surface seawater into the air by splashing water and wind. Nonetheless, the air is a hostile environment to live in due to abiotic stresses like desiccation and ultraviolet radiation. In this study, metagenomics sequencing was used to investigate the air-sea transfer of microorganisms from DNA isolated from marine and air environments. During Ocean Sampling Day (OSD) on 21 June 2015, samples were collected from different coastal sites around Singapore. The samples were sequenced and a set of common species was identified across marine and air samples. They include bacteria species such as Gemmatimonadetes bacterium, Gemmata obscuriglobus and Actinobacterium acAcidi, and fungal species, Rhizophagus irregularis, which are reported in previous studies to have abiotic stress adaptations. In addition, this study will also provide a basis for improvements and optimisation of sampling protocols for OSD 2016.
author2 Enzo Acerbi
author_facet Enzo Acerbi
Lau, Kenny Jia Xu
format Final Year Project
author Lau, Kenny Jia Xu
author_sort Lau, Kenny Jia Xu
title Investigating the relationship between marine and air microbiome in Singapore
title_short Investigating the relationship between marine and air microbiome in Singapore
title_full Investigating the relationship between marine and air microbiome in Singapore
title_fullStr Investigating the relationship between marine and air microbiome in Singapore
title_full_unstemmed Investigating the relationship between marine and air microbiome in Singapore
title_sort investigating the relationship between marine and air microbiome in singapore
publishDate 2016
url http://hdl.handle.net/10356/66996
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