Role of WIP in metastasis and study of transcriptional regulation of N-Wasp

Tumor cell migration and invasion involves actin cytoskeleton reorganization, which is regulated by N-WASP (Neural-Wiskott Aldrich Syndrome Protein) and its interacting proteins such as WASP interacting protein (WIP). Expression of WIP was found to be higher in metastatic cancer cell line compared t...

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Main Author: Amrita S Salvi
Other Authors: Thirumaran s/o Thanabalu
Format: Theses and Dissertations
Language:English
Published: 2016
Subjects:
Online Access:https://hdl.handle.net/10356/67318
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-673182023-02-28T18:45:35Z Role of WIP in metastasis and study of transcriptional regulation of N-Wasp Amrita S Salvi Thirumaran s/o Thanabalu School of Biological Sciences DRNTU::Science::Biological sciences Tumor cell migration and invasion involves actin cytoskeleton reorganization, which is regulated by N-WASP (Neural-Wiskott Aldrich Syndrome Protein) and its interacting proteins such as WASP interacting protein (WIP). Expression of WIP was found to be higher in metastatic cancer cell line compared to its non-metastatic parental cell line. Overexpression of WIP was found to enhance the proliferative, migratory and invasive ability as well as confer anchorage independent growth properties in A549 lung carcinoma cells indicating a pro-metastatic function of WIP. Expression of N-WASP is enhanced in cells undergoing epithelial mesenchymal transition induced by hypoxia. In order to identify mechanism responsible for the differential expression of N-WASP, the N-WASP promoter was characterized, which led to the identification of HRE (Hypoxia Response element) a regulatory region in N-WASP promoter. Our results suggest that transcription factor HiF1α regulates N-WASP expression in promoting metastasis under hypoxic conditions. DOCTOR OF PHILOSOPHY (SBS) 2016-05-15T08:20:33Z 2016-05-15T08:20:33Z 2016 Thesis Amrita S Salvi. (2016). Role of WIP in metastasis and study of transcriptional regulation of N-Wasp. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/67318 10.32657/10356/67318 en 214 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Amrita S Salvi
Role of WIP in metastasis and study of transcriptional regulation of N-Wasp
description Tumor cell migration and invasion involves actin cytoskeleton reorganization, which is regulated by N-WASP (Neural-Wiskott Aldrich Syndrome Protein) and its interacting proteins such as WASP interacting protein (WIP). Expression of WIP was found to be higher in metastatic cancer cell line compared to its non-metastatic parental cell line. Overexpression of WIP was found to enhance the proliferative, migratory and invasive ability as well as confer anchorage independent growth properties in A549 lung carcinoma cells indicating a pro-metastatic function of WIP. Expression of N-WASP is enhanced in cells undergoing epithelial mesenchymal transition induced by hypoxia. In order to identify mechanism responsible for the differential expression of N-WASP, the N-WASP promoter was characterized, which led to the identification of HRE (Hypoxia Response element) a regulatory region in N-WASP promoter. Our results suggest that transcription factor HiF1α regulates N-WASP expression in promoting metastasis under hypoxic conditions.
author2 Thirumaran s/o Thanabalu
author_facet Thirumaran s/o Thanabalu
Amrita S Salvi
format Theses and Dissertations
author Amrita S Salvi
author_sort Amrita S Salvi
title Role of WIP in metastasis and study of transcriptional regulation of N-Wasp
title_short Role of WIP in metastasis and study of transcriptional regulation of N-Wasp
title_full Role of WIP in metastasis and study of transcriptional regulation of N-Wasp
title_fullStr Role of WIP in metastasis and study of transcriptional regulation of N-Wasp
title_full_unstemmed Role of WIP in metastasis and study of transcriptional regulation of N-Wasp
title_sort role of wip in metastasis and study of transcriptional regulation of n-wasp
publishDate 2016
url https://hdl.handle.net/10356/67318
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