Generation of dengue virus non-structural protein 1 antibodies using phage display and hybridoma technology
Dengue Virus (DENV) is an emerging flavivirus that is increasingly endemic worldwide with no efficacious antiviral treatments. Non-Structural Protein 1 (NS1) is an intriguing one that assumes diverse roles during infection ranging from a cofactor in viral replication complex to a disease aggravator....
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| Format: | Final Year Project |
| Language: | English |
| Published: |
2016
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| Online Access: | http://hdl.handle.net/10356/67372 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | Dengue Virus (DENV) is an emerging flavivirus that is increasingly endemic worldwide with no efficacious antiviral treatments. Non-Structural Protein 1 (NS1) is an intriguing one that assumes diverse roles during infection ranging from a cofactor in viral replication complex to a disease aggravator. However, the functions of NS1 are not fully elucidated yet and antibodies would make a useful immunochemical tool to understand it better. Thus, NS1 antibodies are generated using phage display and hybridoma technology. In this project, a chimeric Fab-phage immunized library with a diversity of 4.828 x 10^6 cfu was constructed and screened for strong NS1 Fab-phage binders. Hybridomas produced were also screened for positive NS1 antibody hybridoma producers. As a result, 11 unique clones from phage display and a polyclonal hybridoma culture (2G12) were found. 2G12 is able to bind to NS1 in IFA and seems to bind to NS1 monomer in WB. |
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