Examination of immunological effects of natural products such as porcupine dates extracts (PE) and edible bird’s nest extract (EBN)

Natural products such as porcupine date extracts (PE) and edible bird’s nest (EBN) are believed to have tremendous health benefits that could prevent or even treat illnesses. Hence, with rising rates of diseases, more people are consuming these natural products despite their lack of scientific evide...

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Main Author: Sze, Lynette Shi Jie
Other Authors: Lee Koon Guan
Format: Final Year Project
Language:English
Published: 2016
Subjects:
Online Access:http://hdl.handle.net/10356/67373
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-673732023-02-28T18:02:33Z Examination of immunological effects of natural products such as porcupine dates extracts (PE) and edible bird’s nest extract (EBN) Sze, Lynette Shi Jie Lee Koon Guan School of Biological Sciences A*STAR Bioprocessing Technology Institute DRNTU::Science Natural products such as porcupine date extracts (PE) and edible bird’s nest (EBN) are believed to have tremendous health benefits that could prevent or even treat illnesses. Hence, with rising rates of diseases, more people are consuming these natural products despite their lack of scientific evidences. Here, as part of a collaborative biotechnology industry project, we aim to investigate the antiproliferative properties of various formulations of PE (A-D) by conducting a proliferative assay on lung cancer cells and normal mesenchymal stem cell (MSC) over a 4-week period. We will also study the immunogenic effects of a concoction of EBN with proprietary herb that may confer anti-dengue properties (DVEBN), based on the cytokine induction profile and expression pathway in macrophages and splenocytes via qPCR, RT-PCR and western blot analysis. Our results showed that all but PE B can inhibit lung cancer cells proliferation while not affecting normal cells. We also demonstrated that DVEBN can produce antiviral and anti-inflammatory cytokines and has the ability to inhibit dengue virus replication in macrophages. DVEBN elicits IFNβ expression in both macrophages and splenocytes and we further show that this requires IRF3 activation. Bachelor of Science in Biological Sciences 2016-05-16T05:49:28Z 2016-05-16T05:49:28Z 2016 Final Year Project (FYP) http://hdl.handle.net/10356/67373 en Nanyang Technological University 30 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science
spellingShingle DRNTU::Science
Sze, Lynette Shi Jie
Examination of immunological effects of natural products such as porcupine dates extracts (PE) and edible bird’s nest extract (EBN)
description Natural products such as porcupine date extracts (PE) and edible bird’s nest (EBN) are believed to have tremendous health benefits that could prevent or even treat illnesses. Hence, with rising rates of diseases, more people are consuming these natural products despite their lack of scientific evidences. Here, as part of a collaborative biotechnology industry project, we aim to investigate the antiproliferative properties of various formulations of PE (A-D) by conducting a proliferative assay on lung cancer cells and normal mesenchymal stem cell (MSC) over a 4-week period. We will also study the immunogenic effects of a concoction of EBN with proprietary herb that may confer anti-dengue properties (DVEBN), based on the cytokine induction profile and expression pathway in macrophages and splenocytes via qPCR, RT-PCR and western blot analysis. Our results showed that all but PE B can inhibit lung cancer cells proliferation while not affecting normal cells. We also demonstrated that DVEBN can produce antiviral and anti-inflammatory cytokines and has the ability to inhibit dengue virus replication in macrophages. DVEBN elicits IFNβ expression in both macrophages and splenocytes and we further show that this requires IRF3 activation.
author2 Lee Koon Guan
author_facet Lee Koon Guan
Sze, Lynette Shi Jie
format Final Year Project
author Sze, Lynette Shi Jie
author_sort Sze, Lynette Shi Jie
title Examination of immunological effects of natural products such as porcupine dates extracts (PE) and edible bird’s nest extract (EBN)
title_short Examination of immunological effects of natural products such as porcupine dates extracts (PE) and edible bird’s nest extract (EBN)
title_full Examination of immunological effects of natural products such as porcupine dates extracts (PE) and edible bird’s nest extract (EBN)
title_fullStr Examination of immunological effects of natural products such as porcupine dates extracts (PE) and edible bird’s nest extract (EBN)
title_full_unstemmed Examination of immunological effects of natural products such as porcupine dates extracts (PE) and edible bird’s nest extract (EBN)
title_sort examination of immunological effects of natural products such as porcupine dates extracts (pe) and edible bird’s nest extract (ebn)
publishDate 2016
url http://hdl.handle.net/10356/67373
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