Characterization of the expression pattern of the Not1 regulator of gene expression in the malaria parasite plasmodium falciparum

Malaria is one of the most worrying infectious diseases worldwide. Recent discovery of the artemisinin-resistant Plasmodium species has sparked a great health concern. Studies on stress-induced defense mechanism in these parasites may provide clue on how they survive in multiple host induced stresse...

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Main Author: Tan, Aik Choong
Other Authors: Mark Featherstone
Format: Final Year Project
Language:English
Published: 2016
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Online Access:http://hdl.handle.net/10356/67379
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-673792023-02-28T18:01:03Z Characterization of the expression pattern of the Not1 regulator of gene expression in the malaria parasite plasmodium falciparum Tan, Aik Choong Mark Featherstone School of Biological Sciences DRNTU::Science Malaria is one of the most worrying infectious diseases worldwide. Recent discovery of the artemisinin-resistant Plasmodium species has sparked a great health concern. Studies on stress-induced defense mechanism in these parasites may provide clue on how they survive in multiple host induced stresses. The aim of the research is to investigate the alteration in protein and mRNA level of Not1.1, a component of Ccr4-Not complex, when the parasite is subjected to heat and drug-induced stress. Immunofluorescence Assay (IFA) carried out with FLAG mAb (monoclonal antibody) shows signal in the F-T996; however we also observe a weaker signal in the parent WT strain. This makes the current result inconclusive. We were unable to detect the FLAG-Not1.1 protein in the western blot analysis using FLAG mAb. Generation of monoclonal antibody against Not1.1 may help us address these issues. The existing RT-PCR data is insufficient to conclude the change in mRNA levels. The data in RT-PCR should be validated by including biological replicates and the gene expression analysis should take into account the changes in the expression profile of the Not1.1 across the life cycle under normal condition. By gaining better knowledge on how this organism responds to stressful condition, we might be able to decipher the drug resistance mechanism and develop better Malaria treatment in the future. Bachelor of Science in Biological Sciences 2016-05-16T06:13:30Z 2016-05-16T06:13:30Z 2016 Final Year Project (FYP) http://hdl.handle.net/10356/67379 en Nanyang Technological University 25 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science
spellingShingle DRNTU::Science
Tan, Aik Choong
Characterization of the expression pattern of the Not1 regulator of gene expression in the malaria parasite plasmodium falciparum
description Malaria is one of the most worrying infectious diseases worldwide. Recent discovery of the artemisinin-resistant Plasmodium species has sparked a great health concern. Studies on stress-induced defense mechanism in these parasites may provide clue on how they survive in multiple host induced stresses. The aim of the research is to investigate the alteration in protein and mRNA level of Not1.1, a component of Ccr4-Not complex, when the parasite is subjected to heat and drug-induced stress. Immunofluorescence Assay (IFA) carried out with FLAG mAb (monoclonal antibody) shows signal in the F-T996; however we also observe a weaker signal in the parent WT strain. This makes the current result inconclusive. We were unable to detect the FLAG-Not1.1 protein in the western blot analysis using FLAG mAb. Generation of monoclonal antibody against Not1.1 may help us address these issues. The existing RT-PCR data is insufficient to conclude the change in mRNA levels. The data in RT-PCR should be validated by including biological replicates and the gene expression analysis should take into account the changes in the expression profile of the Not1.1 across the life cycle under normal condition. By gaining better knowledge on how this organism responds to stressful condition, we might be able to decipher the drug resistance mechanism and develop better Malaria treatment in the future.
author2 Mark Featherstone
author_facet Mark Featherstone
Tan, Aik Choong
format Final Year Project
author Tan, Aik Choong
author_sort Tan, Aik Choong
title Characterization of the expression pattern of the Not1 regulator of gene expression in the malaria parasite plasmodium falciparum
title_short Characterization of the expression pattern of the Not1 regulator of gene expression in the malaria parasite plasmodium falciparum
title_full Characterization of the expression pattern of the Not1 regulator of gene expression in the malaria parasite plasmodium falciparum
title_fullStr Characterization of the expression pattern of the Not1 regulator of gene expression in the malaria parasite plasmodium falciparum
title_full_unstemmed Characterization of the expression pattern of the Not1 regulator of gene expression in the malaria parasite plasmodium falciparum
title_sort characterization of the expression pattern of the not1 regulator of gene expression in the malaria parasite plasmodium falciparum
publishDate 2016
url http://hdl.handle.net/10356/67379
_version_ 1759857457371283456