Gelatin-dopamine coating for improved biocompatibility on polydimethylsiloxane substrate surfaces

The current in vitro cell culture technologies of utilizing microfluidic devices has become more common within experimental cell biology. Several decades of heuristic optimization have contributed into perfecting conventional cell culture protocols and devices. In comparison, even for polydime...

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Main Author: Quek, Kai Yun
Other Authors: Wang Dongan
Format: Final Year Project
Language:English
Published: 2016
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Online Access:http://hdl.handle.net/10356/68364
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-683642023-03-03T15:41:31Z Gelatin-dopamine coating for improved biocompatibility on polydimethylsiloxane substrate surfaces Quek, Kai Yun Wang Dongan School of Chemical and Biomedical Engineering BioMedical Engineering Research Centre DRNTU::Engineering The current in vitro cell culture technologies of utilizing microfluidic devices has become more common within experimental cell biology. Several decades of heuristic optimization have contributed into perfecting conventional cell culture protocols and devices. In comparison, even for polydimethylsiloxane (PDMS) being one of the most frequently used material in microfluidic cell culture devices, collective understanding of the differences in cellular behaviour between microfluidic and macroscopic culture is still progressing. Unfortunately, PDMS provides a rigid culture surface as it not favourable for cell culture and it limits the proteins adsorption on PDMS surface. This poses a major challenge in cell biology as the intrinsic high hydrophobicity property implicates incompatibility for long-term cell adhesion, proliferation and directed cell differentiation. Therefore, this study aims to customize improved biocompatible PDMS substratum by developing Gelatin-Dopamine (G-D) fabricating approach to stabilize prolonged mouse embryonic stem cell (mESCs) culture on PDMS surfaces as crosslinks coupled with proteins. G-D PDMS surface analysis revealed modification for wettability, roughness and protein attachment which improved the characteristics of PDMS that determine stem cell fates. Thus, this chemical alteration can enhance the biocompatibility of PDMS, including macro and microfluidic scaled culture systems. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2016-05-25T07:42:48Z 2016-05-25T07:42:48Z 2016 Final Year Project (FYP) http://hdl.handle.net/10356/68364 en Nanyang Technological University 90 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering
spellingShingle DRNTU::Engineering
Quek, Kai Yun
Gelatin-dopamine coating for improved biocompatibility on polydimethylsiloxane substrate surfaces
description The current in vitro cell culture technologies of utilizing microfluidic devices has become more common within experimental cell biology. Several decades of heuristic optimization have contributed into perfecting conventional cell culture protocols and devices. In comparison, even for polydimethylsiloxane (PDMS) being one of the most frequently used material in microfluidic cell culture devices, collective understanding of the differences in cellular behaviour between microfluidic and macroscopic culture is still progressing. Unfortunately, PDMS provides a rigid culture surface as it not favourable for cell culture and it limits the proteins adsorption on PDMS surface. This poses a major challenge in cell biology as the intrinsic high hydrophobicity property implicates incompatibility for long-term cell adhesion, proliferation and directed cell differentiation. Therefore, this study aims to customize improved biocompatible PDMS substratum by developing Gelatin-Dopamine (G-D) fabricating approach to stabilize prolonged mouse embryonic stem cell (mESCs) culture on PDMS surfaces as crosslinks coupled with proteins. G-D PDMS surface analysis revealed modification for wettability, roughness and protein attachment which improved the characteristics of PDMS that determine stem cell fates. Thus, this chemical alteration can enhance the biocompatibility of PDMS, including macro and microfluidic scaled culture systems.
author2 Wang Dongan
author_facet Wang Dongan
Quek, Kai Yun
format Final Year Project
author Quek, Kai Yun
author_sort Quek, Kai Yun
title Gelatin-dopamine coating for improved biocompatibility on polydimethylsiloxane substrate surfaces
title_short Gelatin-dopamine coating for improved biocompatibility on polydimethylsiloxane substrate surfaces
title_full Gelatin-dopamine coating for improved biocompatibility on polydimethylsiloxane substrate surfaces
title_fullStr Gelatin-dopamine coating for improved biocompatibility on polydimethylsiloxane substrate surfaces
title_full_unstemmed Gelatin-dopamine coating for improved biocompatibility on polydimethylsiloxane substrate surfaces
title_sort gelatin-dopamine coating for improved biocompatibility on polydimethylsiloxane substrate surfaces
publishDate 2016
url http://hdl.handle.net/10356/68364
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