Cell charactetization in differnet regions of the native annulus fibrous in a porcine intervetebral disc model through cell sorting and molecular analysis

Low back pain is a health issue worldwide. An estimated 84 % of people are forecasted to experience low back pain during their lifetime. The factors that lead to low back pain are complex, there has been more evidence that shows that intervertebral disc degeneration serves as a major contributor lea...

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Main Author: Teo, Tong
Other Authors: Wang Dongan
Format: Final Year Project
Language:English
Published: 2016
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Online Access:http://hdl.handle.net/10356/68386
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-683862023-03-03T15:40:34Z Cell charactetization in differnet regions of the native annulus fibrous in a porcine intervetebral disc model through cell sorting and molecular analysis Teo, Tong Wang Dongan School of Chemical and Biomedical Engineering Singapore-MIT Alliance for Research and Technology (BioSyM) DRNTU::Engineering::Bioengineering Low back pain is a health issue worldwide. An estimated 84 % of people are forecasted to experience low back pain during their lifetime. The factors that lead to low back pain are complex, there has been more evidence that shows that intervertebral disc degeneration serves as a major contributor leading to the destabilization of the spinal motion segment hence resulting in pain and disability. Current treatments include conservative ones such as the use of nonsteroidal anti-inflammatory drugs (NSAIDs) for short term relief and surgical ones such as spinal fusion to alleviate pain caused by IVD degeneration. Another solution is the use of replacement artificial prosthetic discs which has the ability to suffer from wear and tear , become unstable and hence increasing the risk of spinal cord damage. Hence, there is a need to tap on tissue engineering to build a biological constructs for the replacement of the degenerated tissue. However, the cell biology of the AF is still poorly understood especially the different cell types that exist within the AF. In this study, we will use cell sorting methods and carry out molecular analysis to find out more about the characteristics of the different regions of the native annulus fibrosus in the porcine intervertebral disc model. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2016-05-25T08:32:01Z 2016-05-25T08:32:01Z 2016 Final Year Project (FYP) http://hdl.handle.net/10356/68386 en Nanyang Technological University 63 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Bioengineering
spellingShingle DRNTU::Engineering::Bioengineering
Teo, Tong
Cell charactetization in differnet regions of the native annulus fibrous in a porcine intervetebral disc model through cell sorting and molecular analysis
description Low back pain is a health issue worldwide. An estimated 84 % of people are forecasted to experience low back pain during their lifetime. The factors that lead to low back pain are complex, there has been more evidence that shows that intervertebral disc degeneration serves as a major contributor leading to the destabilization of the spinal motion segment hence resulting in pain and disability. Current treatments include conservative ones such as the use of nonsteroidal anti-inflammatory drugs (NSAIDs) for short term relief and surgical ones such as spinal fusion to alleviate pain caused by IVD degeneration. Another solution is the use of replacement artificial prosthetic discs which has the ability to suffer from wear and tear , become unstable and hence increasing the risk of spinal cord damage. Hence, there is a need to tap on tissue engineering to build a biological constructs for the replacement of the degenerated tissue. However, the cell biology of the AF is still poorly understood especially the different cell types that exist within the AF. In this study, we will use cell sorting methods and carry out molecular analysis to find out more about the characteristics of the different regions of the native annulus fibrosus in the porcine intervertebral disc model.
author2 Wang Dongan
author_facet Wang Dongan
Teo, Tong
format Final Year Project
author Teo, Tong
author_sort Teo, Tong
title Cell charactetization in differnet regions of the native annulus fibrous in a porcine intervetebral disc model through cell sorting and molecular analysis
title_short Cell charactetization in differnet regions of the native annulus fibrous in a porcine intervetebral disc model through cell sorting and molecular analysis
title_full Cell charactetization in differnet regions of the native annulus fibrous in a porcine intervetebral disc model through cell sorting and molecular analysis
title_fullStr Cell charactetization in differnet regions of the native annulus fibrous in a porcine intervetebral disc model through cell sorting and molecular analysis
title_full_unstemmed Cell charactetization in differnet regions of the native annulus fibrous in a porcine intervetebral disc model through cell sorting and molecular analysis
title_sort cell charactetization in differnet regions of the native annulus fibrous in a porcine intervetebral disc model through cell sorting and molecular analysis
publishDate 2016
url http://hdl.handle.net/10356/68386
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