Two-photon fluorescence microscopy of the retina

Retinal diseases and disorders such as Retinal Vein Occlusion, Diabetic Retinopathy, Age-Related Macular Degeneration, and Retinal Tear or Detachments are among the top causes of blindness in the world. Though there are sophisticated technologies available today to diagnose these diseases on a macro...

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Main Author: Garg, Tanisha
Other Authors: Liu Quan
Format: Final Year Project
Language:English
Published: 2016
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Online Access:http://hdl.handle.net/10356/68410
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-684102023-03-03T15:32:17Z Two-photon fluorescence microscopy of the retina Garg, Tanisha Liu Quan School of Chemical and Biomedical Engineering A*STAR SIMTech DRNTU::Engineering::Bioengineering Retinal diseases and disorders such as Retinal Vein Occlusion, Diabetic Retinopathy, Age-Related Macular Degeneration, and Retinal Tear or Detachments are among the top causes of blindness in the world. Though there are sophisticated technologies available today to diagnose these diseases on a macroscopic level, no existing technology enables us to view the microstructures of the retina and understand their physiological changes prior to the onset of, during the development of, and during the treatment of these diseases. A novel technology called two-photon microscopy, which is based on the principle of Confocal Laser Scanning Microscopy and Two-Photon Excitation is being explored for its application in imaging the microstructures of the retina in vivo. This project aims to test the feasibility of imaging the retina using two-photon microscopy in an ex vivo environment. By carrying out two-photon microscopy on specimens of the bovine (pig) retina, prepared in different ways, the microstructures in the retina, more specifically, the photoreceptor cells, could be imaged by detecting their autofluorescence signals. The current challenges that this technology faces in imaging the retina were also understood. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2016-05-25T09:19:43Z 2016-05-25T09:19:43Z 2016 Final Year Project (FYP) http://hdl.handle.net/10356/68410 en Nanyang Technological University 54 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Bioengineering
spellingShingle DRNTU::Engineering::Bioengineering
Garg, Tanisha
Two-photon fluorescence microscopy of the retina
description Retinal diseases and disorders such as Retinal Vein Occlusion, Diabetic Retinopathy, Age-Related Macular Degeneration, and Retinal Tear or Detachments are among the top causes of blindness in the world. Though there are sophisticated technologies available today to diagnose these diseases on a macroscopic level, no existing technology enables us to view the microstructures of the retina and understand their physiological changes prior to the onset of, during the development of, and during the treatment of these diseases. A novel technology called two-photon microscopy, which is based on the principle of Confocal Laser Scanning Microscopy and Two-Photon Excitation is being explored for its application in imaging the microstructures of the retina in vivo. This project aims to test the feasibility of imaging the retina using two-photon microscopy in an ex vivo environment. By carrying out two-photon microscopy on specimens of the bovine (pig) retina, prepared in different ways, the microstructures in the retina, more specifically, the photoreceptor cells, could be imaged by detecting their autofluorescence signals. The current challenges that this technology faces in imaging the retina were also understood.
author2 Liu Quan
author_facet Liu Quan
Garg, Tanisha
format Final Year Project
author Garg, Tanisha
author_sort Garg, Tanisha
title Two-photon fluorescence microscopy of the retina
title_short Two-photon fluorescence microscopy of the retina
title_full Two-photon fluorescence microscopy of the retina
title_fullStr Two-photon fluorescence microscopy of the retina
title_full_unstemmed Two-photon fluorescence microscopy of the retina
title_sort two-photon fluorescence microscopy of the retina
publishDate 2016
url http://hdl.handle.net/10356/68410
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