Comparing the efficiency of Pseudomonas Aeruginosa (PAO1) and Escherichia coli (BL21) to deliver fluorescence in mammalian cancer cells

Cancer has been a prevalent health problem that continues to be fatal largely due to the late diagnosis of cancer and the lack of monitoring of the progress of cancer treatments on patients. Therefore over the years, researchers have been leveraging on the advantages of nanosensors as they have grea...

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Main Author: Nurrashidah Bte Mansoor
Other Authors: Xu Chenjie
Format: Final Year Project
Language:English
Published: 2016
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Online Access:http://hdl.handle.net/10356/68422
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-684222023-03-03T15:37:07Z Comparing the efficiency of Pseudomonas Aeruginosa (PAO1) and Escherichia coli (BL21) to deliver fluorescence in mammalian cancer cells Nurrashidah Bte Mansoor Xu Chenjie School of Chemical and Biomedical Engineering DRNTU::Engineering Cancer has been a prevalent health problem that continues to be fatal largely due to the late diagnosis of cancer and the lack of monitoring of the progress of cancer treatments on patients. Therefore over the years, researchers have been leveraging on the advantages of nanosensors as they have great potential in overcoming those issues. Among the various types of nanosensors, Quantum Dots (QDs) is widely used due to its photostability. However, the current methods of delivering QDs in cells is still regarded as inefficient. Hence many studies aimed to improve the efficiency of the delivery of QDs. Several literatures suggested the use of bacteria to deliver nanoparticles including a study that utilized QD-labelled Pseudomonas Aeruginosa (PAO1). Unfortunately, PAO1 is highly infectious and it is resistant to a variety of antimicrobial substances. Thus there is a need to find a comparable bacteria that can efficiently deliver QDs. This paper aimed to determine whether an Escherichia coli strain, BL21, can be a substitute for PAO1 by comparing the efficiency of BL21 and PAO1 in delivering QDs in mammalian cancer cells. BL21 was chosen as it non-pathogenic and it was used to transfect melanoma cancer cells, B16F10 which was known as a suitable transfection host. Based on the results obtained, QD-labelled BL21 was found to be far more efficient than PAO1 hence making it a competent substitute. Although the findings were in our favour, the experiment conducted could be optimized to further increase the transfection efficiency of BL21 and in vivo studies can be conducted to observe the effects of BL21 on the immune response. Bachelor of Engineering (Chemical and Biomolecular Engineering) 2016-05-26T01:56:44Z 2016-05-26T01:56:44Z 2016 Final Year Project (FYP) http://hdl.handle.net/10356/68422 en Nanyang Technological University 36 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering
spellingShingle DRNTU::Engineering
Nurrashidah Bte Mansoor
Comparing the efficiency of Pseudomonas Aeruginosa (PAO1) and Escherichia coli (BL21) to deliver fluorescence in mammalian cancer cells
description Cancer has been a prevalent health problem that continues to be fatal largely due to the late diagnosis of cancer and the lack of monitoring of the progress of cancer treatments on patients. Therefore over the years, researchers have been leveraging on the advantages of nanosensors as they have great potential in overcoming those issues. Among the various types of nanosensors, Quantum Dots (QDs) is widely used due to its photostability. However, the current methods of delivering QDs in cells is still regarded as inefficient. Hence many studies aimed to improve the efficiency of the delivery of QDs. Several literatures suggested the use of bacteria to deliver nanoparticles including a study that utilized QD-labelled Pseudomonas Aeruginosa (PAO1). Unfortunately, PAO1 is highly infectious and it is resistant to a variety of antimicrobial substances. Thus there is a need to find a comparable bacteria that can efficiently deliver QDs. This paper aimed to determine whether an Escherichia coli strain, BL21, can be a substitute for PAO1 by comparing the efficiency of BL21 and PAO1 in delivering QDs in mammalian cancer cells. BL21 was chosen as it non-pathogenic and it was used to transfect melanoma cancer cells, B16F10 which was known as a suitable transfection host. Based on the results obtained, QD-labelled BL21 was found to be far more efficient than PAO1 hence making it a competent substitute. Although the findings were in our favour, the experiment conducted could be optimized to further increase the transfection efficiency of BL21 and in vivo studies can be conducted to observe the effects of BL21 on the immune response.
author2 Xu Chenjie
author_facet Xu Chenjie
Nurrashidah Bte Mansoor
format Final Year Project
author Nurrashidah Bte Mansoor
author_sort Nurrashidah Bte Mansoor
title Comparing the efficiency of Pseudomonas Aeruginosa (PAO1) and Escherichia coli (BL21) to deliver fluorescence in mammalian cancer cells
title_short Comparing the efficiency of Pseudomonas Aeruginosa (PAO1) and Escherichia coli (BL21) to deliver fluorescence in mammalian cancer cells
title_full Comparing the efficiency of Pseudomonas Aeruginosa (PAO1) and Escherichia coli (BL21) to deliver fluorescence in mammalian cancer cells
title_fullStr Comparing the efficiency of Pseudomonas Aeruginosa (PAO1) and Escherichia coli (BL21) to deliver fluorescence in mammalian cancer cells
title_full_unstemmed Comparing the efficiency of Pseudomonas Aeruginosa (PAO1) and Escherichia coli (BL21) to deliver fluorescence in mammalian cancer cells
title_sort comparing the efficiency of pseudomonas aeruginosa (pao1) and escherichia coli (bl21) to deliver fluorescence in mammalian cancer cells
publishDate 2016
url http://hdl.handle.net/10356/68422
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