Biomarker discovery of thalassemia and sickle-cell disease from blood peptidome
This study aims to discover inherited blood disease biomarkers through peptidomic approaches, in order to develop faster and more economical diagnostic method suitable for population screening. 3 types of inherited blood diseases, thalassemia Hb CS, Hb E and sickle cell disease Hb S were include...
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| Format: | Theses and Dissertations |
| Language: | English |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/68633 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | This study aims to discover inherited blood disease biomarkers through
peptidomic approaches, in order to develop faster and more economical
diagnostic method suitable for population screening. 3 types of inherited blood
diseases, thalassemia Hb CS, Hb E and sickle cell disease Hb S were
included in this study. Hb CS markers were successfully identified through
both MALDI-MS and LC-MS. 2 of the candidate markers were further analyzed
by pseudo-MRM. Pseudo-MRM analysis demonstrated competitive sensitivity
while further reduced total sample analysis time by approximately 25%. Hb E
and Hb S markers were identified through LC-MS but not MALDI using same
approaches. This study successfully identified several promising biomarkers
for 3 different inherited blood diseases, thus provided support for biomarker
discovery through peptidomic/degradomic approaches. Methods developed in
this study could be readily expanded to biomarker discovery of other inherited
blood diseases. Furthermore, as methods are compatible with MRM that is
common in clinical laboratories, this study is readily converted to clinical
applications. |
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