Identification of trimethylated histone H3 lysine 27 binding proteins

Identification of Trimethylated Histone H3 Lysine 27 Binding Proteins We report a novel lysine methyl-binding protein, which selectively recognizes the EZH2-mediated H3K27me3 signal through its C-terminal PHD domain. This novel protein is primarily located in the cytosol and is co-localized with act...

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Main Author: Berger, Hanna Heidi
Other Authors: Su I-Hsin
Format: Theses and Dissertations
Language:English
Published: 2016
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Online Access:http://hdl.handle.net/10356/68858
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-688582023-02-28T18:46:25Z Identification of trimethylated histone H3 lysine 27 binding proteins Berger, Hanna Heidi Su I-Hsin School of Biological Sciences DRNTU::Science::Biological sciences Identification of Trimethylated Histone H3 Lysine 27 Binding Proteins We report a novel lysine methyl-binding protein, which selectively recognizes the EZH2-mediated H3K27me3 signal through its C-terminal PHD domain. This novel protein is primarily located in the cytosol and is co-localized with actin-rich regions. Overexpression of this novel protein leads to increased cell spreading, increased formation of adhesion structures and decreased cell motility. This phenotypic observation is supported by iTRAQ proteomic analysis, RT-qPCR and immunoblotting, which identified multiple cytoskeletal and cell adhesion related proteins to be upregulated. Furthermore, this novel protein partially counteracts the effect of EZH2 on cell spreading. Here, it may either bind to methylated cytosolic proteins or regulate the expression of genes by interacting with H3K27me3. We are currently focusing on the identification of trimethylated proteins that interact with this novel protein. ​Doctor of Philosophy (SBS) 2016-06-14T01:50:30Z 2016-06-14T01:50:30Z 2016 Thesis Berger, H. H. (2016). Identification of trimethylated histone H3 lysine 27 binding proteins. Doctoral thesis, Nanyang Technological University, Singapore. http://hdl.handle.net/10356/68858 en 217 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences
spellingShingle DRNTU::Science::Biological sciences
Berger, Hanna Heidi
Identification of trimethylated histone H3 lysine 27 binding proteins
description Identification of Trimethylated Histone H3 Lysine 27 Binding Proteins We report a novel lysine methyl-binding protein, which selectively recognizes the EZH2-mediated H3K27me3 signal through its C-terminal PHD domain. This novel protein is primarily located in the cytosol and is co-localized with actin-rich regions. Overexpression of this novel protein leads to increased cell spreading, increased formation of adhesion structures and decreased cell motility. This phenotypic observation is supported by iTRAQ proteomic analysis, RT-qPCR and immunoblotting, which identified multiple cytoskeletal and cell adhesion related proteins to be upregulated. Furthermore, this novel protein partially counteracts the effect of EZH2 on cell spreading. Here, it may either bind to methylated cytosolic proteins or regulate the expression of genes by interacting with H3K27me3. We are currently focusing on the identification of trimethylated proteins that interact with this novel protein.
author2 Su I-Hsin
author_facet Su I-Hsin
Berger, Hanna Heidi
format Theses and Dissertations
author Berger, Hanna Heidi
author_sort Berger, Hanna Heidi
title Identification of trimethylated histone H3 lysine 27 binding proteins
title_short Identification of trimethylated histone H3 lysine 27 binding proteins
title_full Identification of trimethylated histone H3 lysine 27 binding proteins
title_fullStr Identification of trimethylated histone H3 lysine 27 binding proteins
title_full_unstemmed Identification of trimethylated histone H3 lysine 27 binding proteins
title_sort identification of trimethylated histone h3 lysine 27 binding proteins
publishDate 2016
url http://hdl.handle.net/10356/68858
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