Investigating the effect of soy isoflavone daidzein on the histone acetylation status of MDA-MB-231 breast cancer cell line

• Main Author: Quek, Su-An
• Other Authors: Koh Cheng Gee
• Format: Final Year Project
• Language: English
• Published: 2016
• Subjects: DRNTU::Science
• Online Access: http://hdl.handle.net/10356/68920
• Institution: Nanyang Technological University

Breast cancer is one of the most common types of cancer among women all over the world. There are many breast cancer subtypes and the prevalence of different types of breast cancer among women of different ethnic background and culture have led scientist to consider how difference in diet can impact the development of different types of breast cancer. Soybean derived isoflavones such as Daidzein have recently become a research target for use in treatment of invasive breast cancer along with other phytoestrogens. Soybeans are commonly used in Asian cuisines especially in Asian countries with lower incidences of invasive breast cancer. The objective of this project was to investigate the effect of Daidzein in increasing Histone acetylation on MDA-MB-231 breast cancer cells. Results from Western Immunoblotting show that acetylation of Histone H3K9 of MDAMB-231 cells increased when treated with Daidzein as compared to the untreated control cells. We demonstrated that concentrations between 50µM to 150µM of Daidzein are the most effective in inducing an increase in histone acetylation at histone H3K9.