Potential of serum in creation of small-diameter vascular graft
With the progression of tissue engineering, decellularization of vascular xenografts has gained increasing attention in hopes to provide affordable off-the-shelf small-diameter vascular grafts (SDVG) for urgent vascular replacements. Previous decellularization studies have shown that both fetal bovi...
Saved in:
Main Author: | |
---|---|
Other Authors: | |
Format: | Final Year Project |
Language: | English |
Published: |
2016
|
Subjects: | |
Online Access: | http://hdl.handle.net/10356/69180 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-69180 |
---|---|
record_format |
dspace |
spelling |
sg-ntu-dr.10356-691802023-02-28T17:59:43Z Potential of serum in creation of small-diameter vascular graft Lim, Wee Keng Thirumaran s/o Thanabalu School of Biological Sciences Department of Plastic, Reconstructive & Aesthetic Surgery, Singapore General Hospital Chan Wing Yue DRNTU::Science With the progression of tissue engineering, decellularization of vascular xenografts has gained increasing attention in hopes to provide affordable off-the-shelf small-diameter vascular grafts (SDVG) for urgent vascular replacements. Previous decellularization studies have shown that both fetal bovine serum (FBS) and human serum (HS) could remove nuclear materials from vascular matrix. However, no current study has compared both sera on their decellularization efficiency. It is also unknown whether matrix-bound growth factors in both sera could influence translatable bioactivity. In this study, we evaluated and compared the decellularization efficiency and bioactivity of decellularized porcine carotid arteries (PCA) from sodium hydroxide (NaOH)-only, FBS- and HS-mediated treatments. Our data suggest a further reduction in DNA and α-gal content from FBS- and HS-mediated decellularized PCA. Between both sera, immunohistochemical analysis indicated more α-gal removal with FBS. Moreover, decellularization with FBS and HS may have an effect on translatable bioactivity without adversely affecting collagen preservation. Together, our preliminary results encourage future studies to further explore the potential of incorporating serum into decellularization protocol for the creation of SDVG. Bachelor of Science in Biological Sciences 2016-11-16T09:09:19Z 2016-11-16T09:09:19Z 2016 Final Year Project (FYP) http://hdl.handle.net/10356/69180 en Nanyang Technological University 37 p. application/pdf |
institution |
Nanyang Technological University |
building |
NTU Library |
continent |
Asia |
country |
Singapore Singapore |
content_provider |
NTU Library |
collection |
DR-NTU |
language |
English |
topic |
DRNTU::Science |
spellingShingle |
DRNTU::Science Lim, Wee Keng Potential of serum in creation of small-diameter vascular graft |
description |
With the progression of tissue engineering, decellularization of vascular xenografts has gained increasing attention in hopes to provide affordable off-the-shelf small-diameter vascular grafts (SDVG) for urgent vascular replacements. Previous decellularization studies have shown that both fetal bovine serum (FBS) and human serum (HS) could remove nuclear materials from vascular matrix. However, no current study has compared both sera on their decellularization efficiency. It is also unknown whether matrix-bound growth factors in both sera could influence translatable bioactivity. In this study, we evaluated and compared the decellularization efficiency and bioactivity of decellularized porcine carotid arteries (PCA) from sodium hydroxide (NaOH)-only, FBS- and HS-mediated treatments. Our data suggest a further reduction in DNA and α-gal content from FBS- and HS-mediated decellularized PCA. Between both sera, immunohistochemical analysis indicated more α-gal removal with FBS. Moreover, decellularization with FBS and HS may have an effect on translatable bioactivity without adversely affecting collagen preservation. Together, our preliminary results encourage future studies to further explore the potential of incorporating serum into decellularization protocol for the creation of SDVG. |
author2 |
Thirumaran s/o Thanabalu |
author_facet |
Thirumaran s/o Thanabalu Lim, Wee Keng |
format |
Final Year Project |
author |
Lim, Wee Keng |
author_sort |
Lim, Wee Keng |
title |
Potential of serum in creation of small-diameter vascular graft |
title_short |
Potential of serum in creation of small-diameter vascular graft |
title_full |
Potential of serum in creation of small-diameter vascular graft |
title_fullStr |
Potential of serum in creation of small-diameter vascular graft |
title_full_unstemmed |
Potential of serum in creation of small-diameter vascular graft |
title_sort |
potential of serum in creation of small-diameter vascular graft |
publishDate |
2016 |
url |
http://hdl.handle.net/10356/69180 |
_version_ |
1759858112575045632 |