Potential of serum in creation of small-diameter vascular graft

With the progression of tissue engineering, decellularization of vascular xenografts has gained increasing attention in hopes to provide affordable off-the-shelf small-diameter vascular grafts (SDVG) for urgent vascular replacements. Previous decellularization studies have shown that both fetal bovi...

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Main Author: Lim, Wee Keng
Other Authors: Thirumaran s/o Thanabalu
Format: Final Year Project
Language:English
Published: 2016
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Online Access:http://hdl.handle.net/10356/69180
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-691802023-02-28T17:59:43Z Potential of serum in creation of small-diameter vascular graft Lim, Wee Keng Thirumaran s/o Thanabalu School of Biological Sciences Department of Plastic, Reconstructive & Aesthetic Surgery, Singapore General Hospital Chan Wing Yue DRNTU::Science With the progression of tissue engineering, decellularization of vascular xenografts has gained increasing attention in hopes to provide affordable off-the-shelf small-diameter vascular grafts (SDVG) for urgent vascular replacements. Previous decellularization studies have shown that both fetal bovine serum (FBS) and human serum (HS) could remove nuclear materials from vascular matrix. However, no current study has compared both sera on their decellularization efficiency. It is also unknown whether matrix-bound growth factors in both sera could influence translatable bioactivity. In this study, we evaluated and compared the decellularization efficiency and bioactivity of decellularized porcine carotid arteries (PCA) from sodium hydroxide (NaOH)-only, FBS- and HS-mediated treatments. Our data suggest a further reduction in DNA and α-gal content from FBS- and HS-mediated decellularized PCA. Between both sera, immunohistochemical analysis indicated more α-gal removal with FBS. Moreover, decellularization with FBS and HS may have an effect on translatable bioactivity without adversely affecting collagen preservation. Together, our preliminary results encourage future studies to further explore the potential of incorporating serum into decellularization protocol for the creation of SDVG. Bachelor of Science in Biological Sciences 2016-11-16T09:09:19Z 2016-11-16T09:09:19Z 2016 Final Year Project (FYP) http://hdl.handle.net/10356/69180 en Nanyang Technological University 37 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science
spellingShingle DRNTU::Science
Lim, Wee Keng
Potential of serum in creation of small-diameter vascular graft
description With the progression of tissue engineering, decellularization of vascular xenografts has gained increasing attention in hopes to provide affordable off-the-shelf small-diameter vascular grafts (SDVG) for urgent vascular replacements. Previous decellularization studies have shown that both fetal bovine serum (FBS) and human serum (HS) could remove nuclear materials from vascular matrix. However, no current study has compared both sera on their decellularization efficiency. It is also unknown whether matrix-bound growth factors in both sera could influence translatable bioactivity. In this study, we evaluated and compared the decellularization efficiency and bioactivity of decellularized porcine carotid arteries (PCA) from sodium hydroxide (NaOH)-only, FBS- and HS-mediated treatments. Our data suggest a further reduction in DNA and α-gal content from FBS- and HS-mediated decellularized PCA. Between both sera, immunohistochemical analysis indicated more α-gal removal with FBS. Moreover, decellularization with FBS and HS may have an effect on translatable bioactivity without adversely affecting collagen preservation. Together, our preliminary results encourage future studies to further explore the potential of incorporating serum into decellularization protocol for the creation of SDVG.
author2 Thirumaran s/o Thanabalu
author_facet Thirumaran s/o Thanabalu
Lim, Wee Keng
format Final Year Project
author Lim, Wee Keng
author_sort Lim, Wee Keng
title Potential of serum in creation of small-diameter vascular graft
title_short Potential of serum in creation of small-diameter vascular graft
title_full Potential of serum in creation of small-diameter vascular graft
title_fullStr Potential of serum in creation of small-diameter vascular graft
title_full_unstemmed Potential of serum in creation of small-diameter vascular graft
title_sort potential of serum in creation of small-diameter vascular graft
publishDate 2016
url http://hdl.handle.net/10356/69180
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