Hevein-like peptides in plants : discovery, characterization and molecular diversity
Hevein-like peptides belong to a family of chitin-binding, cystine-knotted peptides. Based on the number of cysteine residues in hevein-like peptides they can be divided into three sub-classes, namely, 6C-, 8C- and 10C- hevein-like peptides. The objectives of my thesis are to isolate and characteriz...
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sg-ntu-dr.10356-693932023-02-28T18:42:07Z Hevein-like peptides in plants : discovery, characterization and molecular diversity Kini Shruthi Gopalkrishna Luo Qian, Kathy James P. Tam School of Biological Sciences DRNTU::Science::Biological sciences::Botany Hevein-like peptides belong to a family of chitin-binding, cystine-knotted peptides. Based on the number of cysteine residues in hevein-like peptides they can be divided into three sub-classes, namely, 6C-, 8C- and 10C- hevein-like peptides. The objectives of my thesis are to isolate and characterize novel hevein-like peptides and to study their distribution and evolution in plants. From two herbs, Alternanthera sessilis and Moringa oleifera, six novel 6C-hevein-like peptides and three novel 8C-hevein-like peptides were isolated and purified, respectively. In addition, we gained insights into their chitin-binding interaction, anti-fungal activity and biosynthesis pathway. Using an in silico approach, a total of 385 novel hevein-like peptides were found in 124 plant families including bryophytes and gymnosperms. In conclusion, my thesis expands the existing knowledge of hevein-like peptides regarding their structure; function and biosynthesis which may help to develop robust anti-fungal agents and transgenic plants with increased specificity and resistance to fungal infections. DOCTOR OF PHILOSOPHY (SBS) 2016-12-23T04:24:35Z 2016-12-23T04:24:35Z 2016 Thesis Kini Shruthi Gopalkrishna. (2016). Hevein-like peptides in plants : discovery, characterization and molecular diversity. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/69393 10.32657/10356/69393 en 241 p. application/pdf |
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DRNTU::Science::Biological sciences::Botany Kini Shruthi Gopalkrishna Hevein-like peptides in plants : discovery, characterization and molecular diversity |
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Hevein-like peptides belong to a family of chitin-binding, cystine-knotted peptides. Based on the number of cysteine residues in hevein-like peptides they can be divided into three sub-classes, namely, 6C-, 8C- and 10C- hevein-like peptides. The objectives of my thesis are to isolate and characterize novel hevein-like peptides and to study their distribution and evolution in plants. From two herbs, Alternanthera sessilis and Moringa oleifera, six novel 6C-hevein-like peptides and three novel 8C-hevein-like peptides were isolated and purified, respectively. In addition, we gained insights into their chitin-binding interaction, anti-fungal activity and biosynthesis pathway.
Using an in silico approach, a total of 385 novel hevein-like peptides were found in 124 plant families including bryophytes and gymnosperms. In conclusion, my thesis expands the existing knowledge of hevein-like peptides regarding their structure; function and biosynthesis which may help to develop robust anti-fungal agents and transgenic plants with increased specificity and resistance to fungal infections. |
author2 |
Luo Qian, Kathy |
author_facet |
Luo Qian, Kathy Kini Shruthi Gopalkrishna |
format |
Theses and Dissertations |
author |
Kini Shruthi Gopalkrishna |
author_sort |
Kini Shruthi Gopalkrishna |
title |
Hevein-like peptides in plants : discovery, characterization and molecular diversity |
title_short |
Hevein-like peptides in plants : discovery, characterization and molecular diversity |
title_full |
Hevein-like peptides in plants : discovery, characterization and molecular diversity |
title_fullStr |
Hevein-like peptides in plants : discovery, characterization and molecular diversity |
title_full_unstemmed |
Hevein-like peptides in plants : discovery, characterization and molecular diversity |
title_sort |
hevein-like peptides in plants : discovery, characterization and molecular diversity |
publishDate |
2016 |
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https://hdl.handle.net/10356/69393 |
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1759855916134432768 |