Characterization of candidate genes governing complex multicellularity in Neurospora crassa
Complex multicellularity (CM) occurs exclusively in the Eukarya domain and emerged independently within six distinct eukaryotic clades including ascomycete fungi such as Neurospora crassa. All CM organisms are characterized by three-dimensional (3D) cellular organization, cell differentiation and in...
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sg-ntu-dr.10356-705832023-02-28T18:46:35Z Characterization of candidate genes governing complex multicellularity in Neurospora crassa Wong, Jie Yun Mark Stephen Featherstone School of Biological Sciences Temasek Life Sciences Laboratory DRNTU::Science::Biological sciences Complex multicellularity (CM) occurs exclusively in the Eukarya domain and emerged independently within six distinct eukaryotic clades including ascomycete fungi such as Neurospora crassa. All CM organisms are characterized by three-dimensional (3D) cellular organization, cell differentiation and intercellular communication. It was hypothesized that new aspects of CM could be discovered by searching for genes present in CM fungi but absent in unicellular yeast counterparts. To this end, we performed bioinformatics analysis to identify genes expressed in Pezizomycotina and Neolecta irregularis but absent in budding and fission yeast. In total, we identified 147 genes that fit our criteria and we characterized four of which displayed an observable growth defect in N. crassa knockout strains. Interestingly, the protein product of one of the candidate gene, ROGDI, was found to interact with a subunit of the Vacuolar H+-translocating ATPase (V- ATPase) complex as part of the Regulator of ATPase of Vacuoles and Endosomes (RAVE) complex and could potentially function to maintain vacuolar pH levels. Another candidate protein, Spz1, was shown to interact with proteins known to function in cell polarity through its coiled- coil domain. Altogether, the results from this study provide clues on the extant of genes required for CM. Master of Science 2017-05-02T07:46:02Z 2017-05-02T07:46:02Z 2017 Thesis Wong, J. Y. (2017). Characterization of candidate genes governing complex multicellularity in Neurospora crassa. Master's thesis, Nanyang Technological University, Singapore. http://hdl.handle.net/10356/70583 10.32657/10356/70583 en 73 p. application/pdf |
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DRNTU::Science::Biological sciences Wong, Jie Yun Characterization of candidate genes governing complex multicellularity in Neurospora crassa |
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Complex multicellularity (CM) occurs exclusively in the Eukarya domain and emerged independently within six distinct eukaryotic clades including ascomycete fungi such as Neurospora crassa. All CM organisms are characterized by three-dimensional (3D) cellular organization, cell differentiation and intercellular communication. It was hypothesized that new aspects of CM could be discovered by searching for genes present in CM fungi but absent in unicellular yeast counterparts. To this end, we performed bioinformatics analysis to identify genes expressed in Pezizomycotina and Neolecta irregularis but absent in budding and fission yeast. In total, we identified 147 genes that fit our criteria and we characterized four of which displayed an observable growth defect in N. crassa knockout strains. Interestingly, the protein product of one of the candidate gene, ROGDI, was found to interact with a subunit of the Vacuolar H+-translocating ATPase (V- ATPase) complex as part of the Regulator of ATPase of Vacuoles and Endosomes (RAVE) complex and could potentially function to maintain vacuolar pH levels. Another candidate protein, Spz1, was shown to interact with proteins known to function in cell polarity through its coiled- coil domain. Altogether, the results from this study provide clues on the extant of genes required for CM. |
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Mark Stephen Featherstone |
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Mark Stephen Featherstone Wong, Jie Yun |
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Theses and Dissertations |
author |
Wong, Jie Yun |
author_sort |
Wong, Jie Yun |
title |
Characterization of candidate genes governing complex multicellularity in Neurospora crassa |
title_short |
Characterization of candidate genes governing complex multicellularity in Neurospora crassa |
title_full |
Characterization of candidate genes governing complex multicellularity in Neurospora crassa |
title_fullStr |
Characterization of candidate genes governing complex multicellularity in Neurospora crassa |
title_full_unstemmed |
Characterization of candidate genes governing complex multicellularity in Neurospora crassa |
title_sort |
characterization of candidate genes governing complex multicellularity in neurospora crassa |
publishDate |
2017 |
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http://hdl.handle.net/10356/70583 |
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1759857132726910976 |