Mechanical properties of tau pre-mRNA splice site hairpin and its mutants

Understanding the behavior of RNA during the translation and other processes becomes more and more important today since knowledge of biological functions of RNA has increased rapidly. In the last few decades the development of technologies makes the single molecules manipulation much more convenien...

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Main Author: Shi, Jiahao
Other Authors: Chen Gang
Format: Theses and Dissertations
Language:English
Published: 2017
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Online Access:http://hdl.handle.net/10356/71744
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-717442023-02-28T23:32:57Z Mechanical properties of tau pre-mRNA splice site hairpin and its mutants Shi, Jiahao Chen Gang School of Physical and Mathematical Sciences DRNTU::Science::Chemistry Understanding the behavior of RNA during the translation and other processes becomes more and more important today since knowledge of biological functions of RNA has increased rapidly. In the last few decades the development of technologies makes the single molecules manipulation much more convenient and precise, which makes it possible to investigate the relationship between these bio-functions and conformational dynamics of RNA. In this research we mainly focus on studying tau pre-mRNA splice site hairpin and some of its mutants using optical tweezers. Single tau pre-mRNA molecules are combined with DNA/RNA hybrid handles, which can be stretched with optical tweezers, measuring the tiny force around 20 picoNewtons (pN). In this part, we constructed the tau pre-mRNA splice site hairpin flanked by DNA/RNA hybrid handles for single-molecule pulling experiment. Applying Extensible Worm-Like Chain (EWLC) model, we compared the difference of mechanical properties between wild type tau pre-mRNA and some of its mutants, revealing the difference in conformational dynamics between wild type tau pre-mRNA and its mutants. Master of Science 2017-05-19T02:34:05Z 2017-05-19T02:34:05Z 2017 Thesis Shi, J. (2017). Mechanical properties of tau pre-mRNA splice site hairpin and its mutants. Doctoral thesis, Nanyang Technological University, Singapore. http://hdl.handle.net/10356/71744 10.32657/10356/71744 en application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Chemistry
spellingShingle DRNTU::Science::Chemistry
Shi, Jiahao
Mechanical properties of tau pre-mRNA splice site hairpin and its mutants
description Understanding the behavior of RNA during the translation and other processes becomes more and more important today since knowledge of biological functions of RNA has increased rapidly. In the last few decades the development of technologies makes the single molecules manipulation much more convenient and precise, which makes it possible to investigate the relationship between these bio-functions and conformational dynamics of RNA. In this research we mainly focus on studying tau pre-mRNA splice site hairpin and some of its mutants using optical tweezers. Single tau pre-mRNA molecules are combined with DNA/RNA hybrid handles, which can be stretched with optical tweezers, measuring the tiny force around 20 picoNewtons (pN). In this part, we constructed the tau pre-mRNA splice site hairpin flanked by DNA/RNA hybrid handles for single-molecule pulling experiment. Applying Extensible Worm-Like Chain (EWLC) model, we compared the difference of mechanical properties between wild type tau pre-mRNA and some of its mutants, revealing the difference in conformational dynamics between wild type tau pre-mRNA and its mutants.
author2 Chen Gang
author_facet Chen Gang
Shi, Jiahao
format Theses and Dissertations
author Shi, Jiahao
author_sort Shi, Jiahao
title Mechanical properties of tau pre-mRNA splice site hairpin and its mutants
title_short Mechanical properties of tau pre-mRNA splice site hairpin and its mutants
title_full Mechanical properties of tau pre-mRNA splice site hairpin and its mutants
title_fullStr Mechanical properties of tau pre-mRNA splice site hairpin and its mutants
title_full_unstemmed Mechanical properties of tau pre-mRNA splice site hairpin and its mutants
title_sort mechanical properties of tau pre-mrna splice site hairpin and its mutants
publishDate 2017
url http://hdl.handle.net/10356/71744
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