Effect of storage time and effect of sonication on decellularization of porcine esophagus

Esophageal disorders require surgical reconstruction using either alternate substitutes or scaffolds. Tissue engineering have long been touted to be one of the ways to replace diseased or defective organs. This research uses tissue engineering to obtain decellularized esophagus scaffolds. By obtaini...

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Main Author: Pang, Siyao
Other Authors: Chian Kerm Sin
Format: Final Year Project
Language:English
Published: 2017
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Online Access:http://hdl.handle.net/10356/72114
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-721142023-03-04T18:23:37Z Effect of storage time and effect of sonication on decellularization of porcine esophagus Pang, Siyao Chian Kerm Sin School of Mechanical and Aerospace Engineering DRNTU::Engineering::Mechanical engineering Esophageal disorders require surgical reconstruction using either alternate substitutes or scaffolds. Tissue engineering have long been touted to be one of the ways to replace diseased or defective organs. This research uses tissue engineering to obtain decellularized esophagus scaffolds. By obtaining decellularized esophageal tissues, we can accurately maintain the extracellular matrix which provides a suitable microenvironment for recellularization. The aim of this study is to determine if storage time affects decellularization and also if ultrasonic affects decellularization of porcine esophagus. Three set of experiment were done using sample that were kept for 0 days, 11 days and 33 days respectively. The experiment is conducted by soaking porcine esophagus in 1% sodium dodecyl sulfate (SDS) and put into ultrasonic machine which is set to run for 15 minutes and rest for 30 minutes. Histology showed that there is no difference between the samples from different storage time after 3 days, 5 days or 7 days of decellularization done. A control sample of 11 day old esophagus is soaked in 1% SDS and used with a shaker to compare if ultrasonic machine helps decellularization. Histology shows that ultrasonic helps to remove the nuclei from the muscle area faster. We can see this especially after 3 days of decellularization done. For shaking, there are still small amount of nuclei present while for ultrasonic, there is no nuclei present in the muscles area. Therefore, we can conclude that storage time does not affect decellularization and ultrasonic helps decellularization but only confine to the muscle area of the esophagus. Bachelor of Engineering (Mechanical Engineering) 2017-05-26T02:41:18Z 2017-05-26T02:41:18Z 2017 Final Year Project (FYP) http://hdl.handle.net/10356/72114 en Nanyang Technological University 40 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Mechanical engineering
spellingShingle DRNTU::Engineering::Mechanical engineering
Pang, Siyao
Effect of storage time and effect of sonication on decellularization of porcine esophagus
description Esophageal disorders require surgical reconstruction using either alternate substitutes or scaffolds. Tissue engineering have long been touted to be one of the ways to replace diseased or defective organs. This research uses tissue engineering to obtain decellularized esophagus scaffolds. By obtaining decellularized esophageal tissues, we can accurately maintain the extracellular matrix which provides a suitable microenvironment for recellularization. The aim of this study is to determine if storage time affects decellularization and also if ultrasonic affects decellularization of porcine esophagus. Three set of experiment were done using sample that were kept for 0 days, 11 days and 33 days respectively. The experiment is conducted by soaking porcine esophagus in 1% sodium dodecyl sulfate (SDS) and put into ultrasonic machine which is set to run for 15 minutes and rest for 30 minutes. Histology showed that there is no difference between the samples from different storage time after 3 days, 5 days or 7 days of decellularization done. A control sample of 11 day old esophagus is soaked in 1% SDS and used with a shaker to compare if ultrasonic machine helps decellularization. Histology shows that ultrasonic helps to remove the nuclei from the muscle area faster. We can see this especially after 3 days of decellularization done. For shaking, there are still small amount of nuclei present while for ultrasonic, there is no nuclei present in the muscles area. Therefore, we can conclude that storage time does not affect decellularization and ultrasonic helps decellularization but only confine to the muscle area of the esophagus.
author2 Chian Kerm Sin
author_facet Chian Kerm Sin
Pang, Siyao
format Final Year Project
author Pang, Siyao
author_sort Pang, Siyao
title Effect of storage time and effect of sonication on decellularization of porcine esophagus
title_short Effect of storage time and effect of sonication on decellularization of porcine esophagus
title_full Effect of storage time and effect of sonication on decellularization of porcine esophagus
title_fullStr Effect of storage time and effect of sonication on decellularization of porcine esophagus
title_full_unstemmed Effect of storage time and effect of sonication on decellularization of porcine esophagus
title_sort effect of storage time and effect of sonication on decellularization of porcine esophagus
publishDate 2017
url http://hdl.handle.net/10356/72114
_version_ 1759853547162173440