Raman spectroscopy for multiplexed protein detection in tissue bioreactors

• Main Author: Perlaki, Clint Michael
• Other Authors: Sierin Lim
• Format: Theses and Dissertations
• Language: English
• Published: 2017
• Subjects: DRNTU::Science::Chemistry::Biochemistry::Spectroscopy; DRNTU::Engineering::Electrical and electronic engineering::Optics, optoelectronics, photonics; DRNTU::Science::Chemistry::Organic chemistry::Polymers
• Online Access: http://hdl.handle.net/10356/72388
• Institution: Nanyang Technological University

This dissertation presents three major studies utilizing Raman spectroscopy (RS) for the detection and quantification of proteins. All studies were aimed at achieving fast, minimally invasive and cost effective protein detection to potentially complement and eventually replace current methods, such as Western blotting and ELISA, which are labor intensive and slow. Our techniques may have significant effects on biomedical research, particularly with tissue bioreactors, where there is urgent demand for new and fast protein detection technology. In this work, polarized Raman spectroscopy, Raman Optical Activity, and modulated Surface Enhanced Raman Scattering (SERS) were explored.