Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms

Bacterial cells often exist in the form of sessile aggregates known as biofilms. Such biofilms are capable of producing adhesive Extracellular Polymeric Substances (EPS) or matrix. The biofilm matrix is often composed of biopolymers such as polysaccharide, proteins, and extracellular DNA (eDNA). Add...

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Main Author:	Kumaravel Kandaswamy
Other Authors:	Kimberly Kline
Format:	Theses and Dissertations
Language:	English
Published:	2017
Subjects:	DRNTU::Engineering::Chemical engineering
Online Access:	http://hdl.handle.net/10356/72495
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Institution:	Nanyang Technological University
Language:	English

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spelling	sg-ntu-dr.10356-724952021-03-20T13:58:41Z Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms Kumaravel Kandaswamy Kimberly Kline Cohen Yehuda Interdisciplinary Graduate School (IGS) Environmental Engineering Research Centre DRNTU::Engineering::Chemical engineering Bacterial cells often exist in the form of sessile aggregates known as biofilms. Such biofilms are capable of producing adhesive Extracellular Polymeric Substances (EPS) or matrix. The biofilm matrix is often composed of biopolymers such as polysaccharide, proteins, and extracellular DNA (eDNA). Additionally, biofilms also contains phage particles that play a protective role during early stages of biofilm cycle. The matrix of the biofilm is also associated with establishment of chemical gradients, which contributes significantly to the physiological heterogeneity of the biofilm. The aim of this research was to demonstrate the use of fluorescence microscopy techniques to better understand and quantify the spatial organization of proteins and polysaccharides in single cell (Enterococcus faecalis) and biofilm (Pseudomonas aeruginosa) model systems, respectively. In doing so, high-throughput immunofluorescence assay was used to quantify the phenotypic variation in protein localization patterns in hundreds of E. faecalis cells. Furthermore, using Fluorescence Correlation Spectroscopy (FCS) the absolute diffusion coefficients of dextran molecules and phage particles entering P. aeruginosa biofilms was estimated. In essence, this research will provide technical insights on applicability of imaging tools to quantify the process occurring in single cell and biofilm model systems. Doctor of Philosophy (IGS) 2017-08-16T07:39:13Z 2017-08-16T07:39:13Z 2017 Thesis Kumaravel Kandaswamy. (2017). Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms. Doctoral thesis, Nanyang Technological University, Singapore. http://hdl.handle.net/10356/72495 10.32657/10356/72495 en 136 p. application/pdf
institution	Nanyang Technological University
building	NTU Library
continent	Asia
country	Singapore Singapore
content_provider	NTU Library
collection	DR-NTU
language	English
topic	DRNTU::Engineering::Chemical engineering
spellingShingle	DRNTU::Engineering::Chemical engineering Kumaravel Kandaswamy Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms
description	Bacterial cells often exist in the form of sessile aggregates known as biofilms. Such biofilms are capable of producing adhesive Extracellular Polymeric Substances (EPS) or matrix. The biofilm matrix is often composed of biopolymers such as polysaccharide, proteins, and extracellular DNA (eDNA). Additionally, biofilms also contains phage particles that play a protective role during early stages of biofilm cycle. The matrix of the biofilm is also associated with establishment of chemical gradients, which contributes significantly to the physiological heterogeneity of the biofilm. The aim of this research was to demonstrate the use of fluorescence microscopy techniques to better understand and quantify the spatial organization of proteins and polysaccharides in single cell (Enterococcus faecalis) and biofilm (Pseudomonas aeruginosa) model systems, respectively. In doing so, high-throughput immunofluorescence assay was used to quantify the phenotypic variation in protein localization patterns in hundreds of E. faecalis cells. Furthermore, using Fluorescence Correlation Spectroscopy (FCS) the absolute diffusion coefficients of dextran molecules and phage particles entering P. aeruginosa biofilms was estimated. In essence, this research will provide technical insights on applicability of imaging tools to quantify the process occurring in single cell and biofilm model systems.
author2	Kimberly Kline
author_facet	Kimberly Kline Kumaravel Kandaswamy
format	Theses and Dissertations
author	Kumaravel Kandaswamy
author_sort	Kumaravel Kandaswamy
title	Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms
title_short	Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms
title_full	Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms
title_fullStr	Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms
title_full_unstemmed	Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms
title_sort	novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms
publishDate	2017
url	http://hdl.handle.net/10356/72495
_version_	1695706224961519616

Novel imaging approaches to track the movement of proteins and small molecules within bacterial cells and biofilms

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