Influence of five Chinese herbs on bacterial growth, quorum sensing and biofilm formation

Nosocomial pathogens such as Pseudomonas aeruginosa form biofilms to thrive under stress conditions. Biofilm is a community of microorganisms where cells live close to each other and are encased in a protective layer of extracellular polymeric substances against host defenses and antibiotics. Living...

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Main Author: Ang, Jing Yu
Other Authors: Zhao Yan
Format: Final Year Project
Language:English
Published: 2017
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Online Access:http://hdl.handle.net/10356/72510
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-725102023-02-28T18:01:12Z Influence of five Chinese herbs on bacterial growth, quorum sensing and biofilm formation Ang, Jing Yu Zhao Yan School of Biological Sciences Singapore Centre for Environmental Life Sciences Engineering Yang Liang DRNTU::Science::Biological sciences::Microbiology Nosocomial pathogens such as Pseudomonas aeruginosa form biofilms to thrive under stress conditions. Biofilm is a community of microorganisms where cells live close to each other and are encased in a protective layer of extracellular polymeric substances against host defenses and antibiotics. Living in the ‘post-antibiotic era’, many pathologists face issues with drug resistance. Herbal phytochemicals have been shown to exhibit anti-bacteria properties towards pathogenic bacteria. Hence, granule and raw herb form of several herb species (Coptis chinensis, Cortex phellodendri, Scutellaria baicalensis, Salvia miltiorrhiza and Rhizoma zingiberis) were extracted by different solvents and screened for the capacities to reduce growth, quorum sensing (QS) and biofilm formation. Raw Rhizoma zingiberis, Salvia miltiorrhiza, Scutellaria baicalensis, Coptis chinensis and Cortex phellodendri demonstrated capacities in reducing growth and QS at various concentrations: such as 33.3, 50, 100, 100, 50μg/ml. Granule Rhizoma zingiberis, Scutellaria baicalensis and Cortex phellodendri demonstrated capacities in reducing growth at various concentrations: such as 66.7, 100, 66.7μg/ml. Granule Rhizoma zingiberis, Scutellaria baicalensis and Cortex phellodendri demonstrated capacities in reducing QS at various concentrations: such as 16.7, 100, 66.7μg/ml. Raw Rhizoma zingiberis, Salvia miltiorrhiza and granule forms of Rhizoma zingiberis, Salvia miltiorrhiza and Cortex phellodendri demonstrated capacities in reducing biofilm formation at various concentrations: such as 2.1, 50, 4.2, 6.25, 66.7μg/ml. These phenotypic traits of each herb from this study provide guidance towards future studies. Bachelor of Science in Biomedical Sciences 2017-08-17T02:08:45Z 2017-08-17T02:08:45Z 2017 Final Year Project (FYP) http://hdl.handle.net/10356/72510 en Nanyang Technological University 45 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Microbiology
spellingShingle DRNTU::Science::Biological sciences::Microbiology
Ang, Jing Yu
Influence of five Chinese herbs on bacterial growth, quorum sensing and biofilm formation
description Nosocomial pathogens such as Pseudomonas aeruginosa form biofilms to thrive under stress conditions. Biofilm is a community of microorganisms where cells live close to each other and are encased in a protective layer of extracellular polymeric substances against host defenses and antibiotics. Living in the ‘post-antibiotic era’, many pathologists face issues with drug resistance. Herbal phytochemicals have been shown to exhibit anti-bacteria properties towards pathogenic bacteria. Hence, granule and raw herb form of several herb species (Coptis chinensis, Cortex phellodendri, Scutellaria baicalensis, Salvia miltiorrhiza and Rhizoma zingiberis) were extracted by different solvents and screened for the capacities to reduce growth, quorum sensing (QS) and biofilm formation. Raw Rhizoma zingiberis, Salvia miltiorrhiza, Scutellaria baicalensis, Coptis chinensis and Cortex phellodendri demonstrated capacities in reducing growth and QS at various concentrations: such as 33.3, 50, 100, 100, 50μg/ml. Granule Rhizoma zingiberis, Scutellaria baicalensis and Cortex phellodendri demonstrated capacities in reducing growth at various concentrations: such as 66.7, 100, 66.7μg/ml. Granule Rhizoma zingiberis, Scutellaria baicalensis and Cortex phellodendri demonstrated capacities in reducing QS at various concentrations: such as 16.7, 100, 66.7μg/ml. Raw Rhizoma zingiberis, Salvia miltiorrhiza and granule forms of Rhizoma zingiberis, Salvia miltiorrhiza and Cortex phellodendri demonstrated capacities in reducing biofilm formation at various concentrations: such as 2.1, 50, 4.2, 6.25, 66.7μg/ml. These phenotypic traits of each herb from this study provide guidance towards future studies.
author2 Zhao Yan
author_facet Zhao Yan
Ang, Jing Yu
format Final Year Project
author Ang, Jing Yu
author_sort Ang, Jing Yu
title Influence of five Chinese herbs on bacterial growth, quorum sensing and biofilm formation
title_short Influence of five Chinese herbs on bacterial growth, quorum sensing and biofilm formation
title_full Influence of five Chinese herbs on bacterial growth, quorum sensing and biofilm formation
title_fullStr Influence of five Chinese herbs on bacterial growth, quorum sensing and biofilm formation
title_full_unstemmed Influence of five Chinese herbs on bacterial growth, quorum sensing and biofilm formation
title_sort influence of five chinese herbs on bacterial growth, quorum sensing and biofilm formation
publishDate 2017
url http://hdl.handle.net/10356/72510
_version_ 1759855916803424256