Optimization and isolation of bioactive natural products from sediment actinomycetes

Alarming numbers of microorganisms are gaining multi-drug resistance in hospital and community settings, thereby presenting urgency to combat this issue. This study aimed to discover and characterize bioactive secondary metabolites produced by 2 strains of actinomycetes namely P19 and SD50, which we...

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Main Author: Boo, Charlotte Ai-En
Other Authors: Liang Zhao-Xun
Format: Final Year Project
Language:English
Published: 2017
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Online Access:http://hdl.handle.net/10356/72511
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-725112023-02-28T18:01:11Z Optimization and isolation of bioactive natural products from sediment actinomycetes Boo, Charlotte Ai-En Liang Zhao-Xun School of Biological Sciences DRNTU::Science Alarming numbers of microorganisms are gaining multi-drug resistance in hospital and community settings, thereby presenting urgency to combat this issue. This study aimed to discover and characterize bioactive secondary metabolites produced by 2 strains of actinomycetes namely P19 and SD50, which were derived from quarry and mangrove sources respectively. P19 strain was cultured in Pharmamedia and growth conditions were monitored with different flasks. SD50 was cultured on MS agar plates prior to the extraction. Analysis of P19 and SD50 ethyl acetate and acetone extracts using analytical High-Performance Liquid Chromatography (HPLC) and subsequent fractionation of extracts using semi-preparative HPLC successfully isolated compounds used for bioactivity assays. Strong bioactivities displaying more than 90% inhibition were found in P19 pure compound with broad-spectrum antibacterial properties; SD50 fraction C with antibacterial and antifungal properties and SD50 yellow compound D with antibacterial properties. Mass Spectrometry of P19 pure compound has revealed that it is a possible analogue of echinomycin and SD50 yellow compound D to belong to the same class of antibiotics as anthracycline or angucycline. Future research incorporating Nuclear Magnetic Resonance spectroscopy could identify these potentially novel compounds. Moreover, expression of encrypted metabolites in strains P19 and SD50 under different conditions could be attempted. Bachelor of Science in Biomedical Sciences 2017-08-17T02:16:04Z 2017-08-17T02:16:04Z 2017 Final Year Project (FYP) http://hdl.handle.net/10356/72511 en Nanyang Technological University 33 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science
spellingShingle DRNTU::Science
Boo, Charlotte Ai-En
Optimization and isolation of bioactive natural products from sediment actinomycetes
description Alarming numbers of microorganisms are gaining multi-drug resistance in hospital and community settings, thereby presenting urgency to combat this issue. This study aimed to discover and characterize bioactive secondary metabolites produced by 2 strains of actinomycetes namely P19 and SD50, which were derived from quarry and mangrove sources respectively. P19 strain was cultured in Pharmamedia and growth conditions were monitored with different flasks. SD50 was cultured on MS agar plates prior to the extraction. Analysis of P19 and SD50 ethyl acetate and acetone extracts using analytical High-Performance Liquid Chromatography (HPLC) and subsequent fractionation of extracts using semi-preparative HPLC successfully isolated compounds used for bioactivity assays. Strong bioactivities displaying more than 90% inhibition were found in P19 pure compound with broad-spectrum antibacterial properties; SD50 fraction C with antibacterial and antifungal properties and SD50 yellow compound D with antibacterial properties. Mass Spectrometry of P19 pure compound has revealed that it is a possible analogue of echinomycin and SD50 yellow compound D to belong to the same class of antibiotics as anthracycline or angucycline. Future research incorporating Nuclear Magnetic Resonance spectroscopy could identify these potentially novel compounds. Moreover, expression of encrypted metabolites in strains P19 and SD50 under different conditions could be attempted.
author2 Liang Zhao-Xun
author_facet Liang Zhao-Xun
Boo, Charlotte Ai-En
format Final Year Project
author Boo, Charlotte Ai-En
author_sort Boo, Charlotte Ai-En
title Optimization and isolation of bioactive natural products from sediment actinomycetes
title_short Optimization and isolation of bioactive natural products from sediment actinomycetes
title_full Optimization and isolation of bioactive natural products from sediment actinomycetes
title_fullStr Optimization and isolation of bioactive natural products from sediment actinomycetes
title_full_unstemmed Optimization and isolation of bioactive natural products from sediment actinomycetes
title_sort optimization and isolation of bioactive natural products from sediment actinomycetes
publishDate 2017
url http://hdl.handle.net/10356/72511
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