Rapid characterisation and detection of Aspergillus fumigatus and Aspergillus flavus from environmental air samples

Aspergillus causes life-threatening infections in certain at-risk patient populations, and is linked to millions of asthma or other allergic syndromes each year. Though there is fair understanding of Aspergillus detection from clinical specimens, little has been published on detection of airborne...

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Main Author: Chiew, Jean Ling
Other Authors: Yap Peng Huat Eric
Format: Final Year Project
Language:English
Published: 2017
Subjects:
Online Access:http://hdl.handle.net/10356/72625
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-726252020-11-01T05:32:48Z Rapid characterisation and detection of Aspergillus fumigatus and Aspergillus flavus from environmental air samples Chiew, Jean Ling Yap Peng Huat Eric Lee Kong Chian School of Medicine (LKCMedicine) DRNTU::Science::Medicine Aspergillus causes life-threatening infections in certain at-risk patient populations, and is linked to millions of asthma or other allergic syndromes each year. Though there is fair understanding of Aspergillus detection from clinical specimens, little has been published on detection of airborne Aspergillus to assess risk of exposure from spores in Singapore’s urban indoor environment. Furthermore, while Polymerase Chain Reaction techniques have proven high sensitivity and specificity over culture-based methods for detecting Aspergillus, they lack global standardisation and hence are not routinely used clinically. When used, traditional methods such as conventional or Real-Time PCR (RTPCR) take up to hours to detect presence of Aspergillus, potentially delaying diagnosis or treatment. As such, this paper’s objective is “rapid characterisation and detection of Aspergillus fumigatus and Aspergillus flavus from environmental samples”. This paper has succeeded in using conventional PCR and RT-PCR to detect and identify Aspergillus fumigatus and Aspergillus flavus from environmental air samples of significance to Singaporean students, working and laboratory personnel. Samples were identified successfully both at genus and species level, analysed using gel electrophoresis and melt curves. We have also successfully developed Fast PCR equipment and techniques to amplify Aspergillus in under 10 minutes. Detection is complemented by gel electrophoresis and fluorescence comparisons preand post-Fast PCR. With further development, this new technology can prospectively be used for rapid Aspergillus detection in clinical specimens for point-of-care diagnostics, aiding diagnosis and treatment in a bid to reduce Aspergillus-related mortality and morbidity. Bachelor of Medicine and Bachelor of Surgery 2017-08-30T08:36:07Z 2017-08-30T08:36:07Z 2017 Final Year Project (FYP) http://hdl.handle.net/10356/72625 en 25 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Medicine
spellingShingle DRNTU::Science::Medicine
Chiew, Jean Ling
Rapid characterisation and detection of Aspergillus fumigatus and Aspergillus flavus from environmental air samples
description Aspergillus causes life-threatening infections in certain at-risk patient populations, and is linked to millions of asthma or other allergic syndromes each year. Though there is fair understanding of Aspergillus detection from clinical specimens, little has been published on detection of airborne Aspergillus to assess risk of exposure from spores in Singapore’s urban indoor environment. Furthermore, while Polymerase Chain Reaction techniques have proven high sensitivity and specificity over culture-based methods for detecting Aspergillus, they lack global standardisation and hence are not routinely used clinically. When used, traditional methods such as conventional or Real-Time PCR (RTPCR) take up to hours to detect presence of Aspergillus, potentially delaying diagnosis or treatment. As such, this paper’s objective is “rapid characterisation and detection of Aspergillus fumigatus and Aspergillus flavus from environmental samples”. This paper has succeeded in using conventional PCR and RT-PCR to detect and identify Aspergillus fumigatus and Aspergillus flavus from environmental air samples of significance to Singaporean students, working and laboratory personnel. Samples were identified successfully both at genus and species level, analysed using gel electrophoresis and melt curves. We have also successfully developed Fast PCR equipment and techniques to amplify Aspergillus in under 10 minutes. Detection is complemented by gel electrophoresis and fluorescence comparisons preand post-Fast PCR. With further development, this new technology can prospectively be used for rapid Aspergillus detection in clinical specimens for point-of-care diagnostics, aiding diagnosis and treatment in a bid to reduce Aspergillus-related mortality and morbidity.
author2 Yap Peng Huat Eric
author_facet Yap Peng Huat Eric
Chiew, Jean Ling
format Final Year Project
author Chiew, Jean Ling
author_sort Chiew, Jean Ling
title Rapid characterisation and detection of Aspergillus fumigatus and Aspergillus flavus from environmental air samples
title_short Rapid characterisation and detection of Aspergillus fumigatus and Aspergillus flavus from environmental air samples
title_full Rapid characterisation and detection of Aspergillus fumigatus and Aspergillus flavus from environmental air samples
title_fullStr Rapid characterisation and detection of Aspergillus fumigatus and Aspergillus flavus from environmental air samples
title_full_unstemmed Rapid characterisation and detection of Aspergillus fumigatus and Aspergillus flavus from environmental air samples
title_sort rapid characterisation and detection of aspergillus fumigatus and aspergillus flavus from environmental air samples
publishDate 2017
url http://hdl.handle.net/10356/72625
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