In-vitro chondrogenesis and hypertrophy of implanted cells in specific template

• Main Author: Chia, Nang Ying
• Other Authors: Wang Dongan
• Format: Final Year Project
• Language: English
• Published: 2018
• Subjects: DRNTU::Engineering::Bioengineering
• Online Access: http://hdl.handle.net/10356/75128
• Institution: Nanyang Technological University

Bone has the ability to undergo self-healing process when bone fracture occurs. However, self-healing process is only applicable for minor fractures. Major bone fracture’s recovery does not depend on self-healing process. Living hyaline cartilaginous graft (LhCG) which has some similar properties to native hyaline cartilage: could excellently serve as an in-vitro platform that encourages endochondral ossification process. in which it encourages osteogenic progenitors in undergoing proliferation and differentiation. In this experimental study, LhCG was investigated. It is basically a scaffold-free tissue constructs and consists of only their extracellular matrix (ECM) and living chondrocytes. Decellularization was performed to remove its chondrocyte content and become decellularized living hyaline cartilaginous graft (dLhCG). To assess the effectiveness of dLhCG as a viable platform for the formation of bone, mouse mesenchymal stem cells (mMSCs) were seeded onto the dLhCG with different set of cell numbers. On the other hand, another set of experiment, dLhCG coated with Polydopamine (PDA) with different PDA concentration and immersion time were performed to investigate the effectiveness of PDA in enhancing cell adhesion stability onto the dLhCG. The results showed that PDA could help to enhance the cell adhesion stability on the dLhCG with appropriate concentration and immersion time. Apart from that, by seeding different cell numbers into the dLhCG, the results showed that different cells numbers could also affect the cell viability.