Developing whole cell kInetic model

• Main Author: Tan, Daniel Fook Hao
• Other Authors: Liu Quan
• Format: Final Year Project
• Language: English
• Published: 2018
• Subjects: DRNTU::Engineering::Bioengineering; DRNTU::Science::Chemistry::Physical chemistry::Reactions and kinetics; DRNTU::Science::Biological sciences::Molecular biology
• Online Access: http://hdl.handle.net/10356/75199
• Institution: Nanyang Technological University

Computational strain design models have been created to aid the field of metabolic engineering, which has been advanced due to genome editing and copying techniques. Opting the kinetic model instead of the stoichiometric model in their 2014 paper, Khodayari and Maranas managed to create a model which can be used to stimulate E.coli environments before laboratory experiments that can potentially be used to detect changes into the host environments upon introduction of a foreign metabolic pathway. In this report, the original model was extended and improved to achieve a higher included metabolite count and higher accuracy.