Microneedles for diagnosis and therapeutic drug monitoring
The first invention of glucose biosensor was made by Leland Clark in the mid of 20th century. He was considered as the ‘father of biosensor’ due to his discovery of the fundamental principal of a glucose biosensor, which is widely used today for diabetic diagnosis. A variety of glucose sensing de...
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| Format: | Final Year Project |
| Language: | English |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/75205 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | The first invention of glucose biosensor was made by Leland Clark in the mid of 20th
century. He was considered as the ‘father of biosensor’ due to his discovery of the
fundamental principal of a glucose biosensor, which is widely used today for diabetic
diagnosis. A variety of glucose sensing devices have been introduced to the market
nowadays with the aim of efficiently monitoring glucose level in a convenient, low cost
and least uncomfortable feeling way. This study investigates the reliability used of
paper-based MeHA microneedle patches penetrating through the agarose gel to detect
the presence of glucose. The microneedles were fabricated with addition of the polymer
MeHA into the casted PDMS mold. A solution containing enzymes and color indicators
for glucose detection was prepared and coated on nitrocellulose paper. The paper was
immediately attached on the dried microneedle patch due to the good hydrophilicity of
the MeHA for better adhesion. The fabrication of microneedle was followed by the
characterization on its mechanical strength. The MeHA microneedle used in this project
exhibits its capability in sustaining its structural integrity under the execution of 0.1N,
which is the insertion force to the skin. The colorimetric detection of glucose substance
with MeHA microneedle was analysed by converting the R, G, B form of data into
saturation and value. Results showed that the saturation and value vary along with the
glucose concentration present in the environment. This technology can be further
advanced by investigating its multi-analyte capability, specificity and cell cytotoxicity
to establish a reliable, safe and convenient biosensor in the future. |
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